PXD001276

PXD001276 is an original dataset announced via ProteomeXchange.

Title	Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells
Description	Multiple myeloma RPMI8226 cells adapted to growth in melphalan display a shift towards Warburg metabolism and modulated oxidative stress signaling. Inhibitors targeting specific enzymes in these pathways are selectively toxic to the melphalan-resistant cells. To investigate large scale alterations in gene expression accompanying melphalan resistance, we used the multiple myeloma cell line RPMI8226 and its melphalan-resistant derivative LR5. The stable isotope labeling by amino acids in cell culture (SILAC) approach.
HostingRepository	PRIDE
AnnounceDate	2015-05-26
AnnouncementXML	Submission_2015-05-26_07:22:39.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Animesh Sharma
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-09-01 03:22:46	ID requested
⏵ 1	2015-05-26 07:22:40	announced

Zub KA, Sousa MM, Sarno A, Sharma A, Demirovic A, Rao S, Young C, Aas PA, Ericsson I, Sundan A, Jensen ON, Slupphaug G, Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells. PLoS One, 10(3):e0119857(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: SILAC, Multiple myeloma, RPMI8226, melphalan, Warburg, metabolism, oxidative, stress, signaling

Geir Slupphaug
contact affiliation	Professor at Department of Cancer Research and Molecular Medicine Faculty of Medicine Laboratory Centre, 5th floor. Erling Skjalgssons gt. 1 Norwegian University of Science and Technology NTNU 7006 Trondheim, Norway
contact email	geir.slupphaug@ntnu.no
lab head
Animesh Sharma
contact affiliation	Engineer at NTNU, Norway
contact email	sharma.animesh@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001276

PRIDE project URI

• PRIDE
  1. PXD001276
     1. Label: PRIDE project
     2. Name: Modulation of cell metabolic pathways and oxidative stress signaling contribute to acquired melphalan resistance in multiple myeloma cells