PXD001273

PXD001273 is an original dataset announced via ProteomeXchange.

Title	Histone H3 tail interactome in MPC11 murine myeloma cells
Description	We have used peptide capture in conjunction with super-SILAC quantification to carry out an unbiased high-throughput analysis of the composition of protein complexes that bind to histone H3K9/S10 and H3K27/S28 methyl-phospho modifications. We compared binding profiles of nuclear proteins from MPC11 myeloma cells to peptides carrying the H3K9me3, H3K27me3, H3S10ph, H3S28ph and the double H3K9me3/S10ph and H3K27me3/S28ph modifications. The analysis was carried out using peptides corresponding to amino acids 1-20 and 18-38 and the unmodified peptides were also analysed to assess binding to the unmodified histone tail. In order to ensure that the proteins present in any of the "heavy" capture assay samples have corresponding peptides in the common "light" internal reference, we adapted the super-SILAC approach using a reference sample that was obtained by mixing the 8 pulldowns and the negative beads-only control. The method allows us to accurately quantify binding and directly compare levels of binding to peptides that carry a number of different methyl-phospho combinations. This makes it possible for use indeatiled bioinformatic analyses.
HostingRepository	PRIDE
AnnounceDate	2016-05-04
AnnouncementXML	Submission_2016-05-04_07:08:24.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Kunowska
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue; deamidated L-asparagine; deamidated L-glutamine; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-28 07:38:08	ID requested
⏵ 1	2016-05-04 07:08:26	announced

Kunowska N, Rotival M, Yu L, Choudhary J, Dillon N, Identification of protein complexes that bind to histone H3 combinatorial modifications using super-SILAC and weighted correlation network analysis. Nucleic Acids Res, 43(3):1418-32(2015) [pubmed]

curator keyword: Biological, Technical

submitter keyword: histone H3 tail interactome, histone modification, peptide capture, LC-MSMS

Niall Dillon
contact affiliation	MRC Clinical Sciences Centre, Gene Regulation and Chromatin Group, London, UK
contact email	niall.dillom@csc.mrc.ac.uk
lab head
Natalia Kunowska
contact affiliation	MRC Clinical Sciences Centre
contact email	natalia.kunowska@csc.mrc.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001273
     1. Label: PRIDE project
     2. Name: Histone H3 tail interactome in MPC11 murine myeloma cells