PXD001271

PXD001271 is an original dataset announced via ProteomeXchange.

Title	HT-29 treated with mature fragilysin LC-MSMS
Description	Fragilysin is a protein secreted by toxigenic Bacteroides fragilis strains. Fragilysin has a motive HEXXHXXGXXH, which is a zinc-binding motif found in metalloproteinases clan termed metzincins. In this study we obtained all three known recombinant fragilysin isoforms in Escherichia coli and tested its activity. We detected no cleavage of gelatin and chromogenic substrates (such as azocoll and azocasein) by all isoforms. We demonstrated that treatment of HT-29 cells with fragilysins caused E-cadherin cleavage, nevertheless cleavage of recombinant E-cadherins was not observe as well as E-cadherin in isolated cell fractions. It was shown that the native structure of active site characteristic for metalloproteinases is necessary for fragilysin biologic activity. We detected proteins that are released in cultural medium after HT-29 cells treatment with fragilysin. These proteins are potential substrates for fragilysin.
HostingRepository	PRIDE
AnnounceDate	2015-05-26
AnnouncementXML	Submission_2015-05-26_07:07:02.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001271
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ilya Altukhov
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; S-methyl-L-methionine
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-28 01:28:52	ID requested
⏵ 1	2015-05-26 07:07:03	announced

Kharlampieva D, Manuvera V, Podgorny O, Grafskaia E, Kovalchuk S, Pobeguts O, Altukhov I, Govorun V, Lazarev V, Recombinant fragilysin isoforms cause E-cadherin cleavage of intact cells and do not cleave isolated E-cadherin. Microb Pathog, 83-84():47-56(2015) [pubmed]

curator keyword: Biological

submitter keyword: HT-29, fragilysin, BFT

Vasiliy Lazarev
contact affiliation	D.Sc., associate professor Head of Laboratory of Genetic Engineering Research Institute of Physical-Chemical Medicine
contact email	lazarev@niifhm.ru
lab head
Ilya Altukhov
contact affiliation	Research Institute of Physical Chemical Medicin
contact email	ilya.altukhov@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001271

PRIDE project URI

• PRIDE
  1. PXD001271
     1. Label: PRIDE project
     2. Name: HT-29 treated with mature fragilysin LC-MSMS