PXD001270

PXD001270 is an original dataset announced via ProteomeXchange.

Title	Identification of immunodominant antigen among Brucella species (B. abortus and B. melitensis) using hyper immune serum from naturally infected hosts
Description	In the present study, the whole cell protein extracts of B. abortus and B. melitensis were separated using SDS-PAGE and western blotting was carried out with the antiserum from naturally infected host animals (cow, buffalo, goat and sheep). The proteins bands that matched with western blot signals were excised, trypsin digested and subjected to MALDI identification.
HostingRepository	PRIDE
AnnounceDate	2014-12-01
AnnouncementXML	Submission_2015-03-26_06:13:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001270
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jayaseelan Murugaiyan
SpeciesList	scientific name: Brucella melitensis; NCBI TaxID: 29459; scientific name: Brucella abortus; NCBI TaxID: 235; scientific name: Brucella; NCBI TaxID: 234;
ModificationList	monohydroxylated residue
Instrument	ultraflex

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-27 02:48:20	ID requested
1	2014-12-01 02:02:08	announced
⏵ 2	2015-03-26 06:13:35	announced	Updated publication reference for PubMed record(s): 25446124.

Wareth G, Melzer F, Weise C, Neubauer H, Roesler U, Murugaiyan J, Proteomics-based identification of immunodominant proteins of Brucellae using sera from infected hosts points towards enhanced pathogen survival during the infection. Biochem Biophys Res Commun, 456(1):202-6(2015) [pubmed]

curator keyword: Biological

submitter keyword: Brucella, Western blotting, MALDI TOF MS, Immunodominat proteins, hyper immune serum

Jayaseelan Murugaiyan
contact affiliation	Institute of Animal Hygiene and Environmental Health, Centre for Infections Medicine, Freie Universitaet-Berlin, Robert-von-Ostertag Str. 7 – 13, 14163 Berlin, Germany
contact email	jayaseelan.murugaiyan@fu-berlin.de
lab head
Jayaseelan Murugaiyan
contact affiliation	Freie Universitaet-Berlin,Institute of Animal Hygiene and Environmental Health
contact email	jayaseelan.murugaiyan@fu-berlin.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/12/PXD001270

PRIDE project URI

• PRIDE
  1. PXD001270
     1. Label: PRIDE project
     2. Name: Identification of immunodominant antigen among Brucella species (B. abortus and B. melitensis) using hyper immune serum from naturally infected hosts