PXD001269 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Investigating macrophage multinucleation by LC-MS/MS |
Description | Macrophage multinucleation (MM) is essential for various biological processes such as osteoclast-mediated bone resorption and multinucleated giant cell-associated inflammatory reactions. Here we study the molecular pathways underlying multinucleation in the rat through an integrative approach combining MS-based quantitative phosphoproteomics and transcriptome (high throughput RNA-sequencing) to identify new regulators of MM. We show that a strong metabolic shift towards HIF1-mediated glycolysis occurs at transcriptomic level during MM, together with modifications in phosphorylation of over 50 proteins including several ARF GTPase activators and polyphosphate inositol phosphatases. These results will provide a new framework for the combined analysis of transcriptional and post-translational changes during macrophage multinucleation, prioritizing essential genes and revealing the sequential events leading to the multinucleation of macrophages. |
HostingRepository | PRIDE |
AnnounceDate | 2016-07-06 |
AnnouncementXML | Submission_2016-07-06_05:21:22.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001269 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Peter Faull |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | monohydroxylated residue; dehydrated residue; iodoacetamide derivatized residue; phosphorylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-27 01:45:04 | ID requested | |
⏵ 1 | 2016-07-06 05:21:24 | announced | |
Publication List
Rotival M, Ko JH, Srivastava PK, Kerloc'h A, Montoya A, Mauro C, Faull P, Cutillas PR, Petretto E, Behmoaras J, Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation. Mol Cell Proteomics, 14(3):484-98(2015) [pubmed] |
Keyword List
curator keyword: Biomedical, Biological |
submitter keyword: Macrophage, WKY, protein, rat |
Contact List
Peter Allen Faull |
contact affiliation | MRC Clinical Sciences Centre |
contact email | peter.faull@csc.mrc.ac.uk |
lab head | |
Peter Faull |
contact affiliation | MRC Clinical Sciences Centre |
contact email | peter.faull@csc.mrc.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001269
- Label: PRIDE project
- Name: Investigating macrophage multinucleation by LC-MS/MS