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PXD001269

PXD001269 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleInvestigating macrophage multinucleation by LC-MS/MS
DescriptionMacrophage multinucleation (MM) is essential for various biological processes such as osteoclast-mediated bone resorption and multinucleated giant cell-associated inflammatory reactions. Here we study the molecular pathways underlying multinucleation in the rat through an integrative approach combining MS-based quantitative phosphoproteomics and transcriptome (high throughput RNA-sequencing) to identify new regulators of MM. We show that a strong metabolic shift towards HIF1-mediated glycolysis occurs at transcriptomic level during MM, together with modifications in phosphorylation of over 50 proteins including several ARF GTPase activators and polyphosphate inositol phosphatases. These results will provide a new framework for the combined analysis of transcriptional and post-translational changes during macrophage multinucleation, prioritizing essential genes and revealing the sequential events leading to the multinucleation of macrophages.
HostingRepositoryPRIDE
AnnounceDate2016-07-06
AnnouncementXMLSubmission_2016-07-06_05:21:22.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001269
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterPeter Faull
SpeciesList scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationListmonohydroxylated residue; dehydrated residue; iodoacetamide derivatized residue; phosphorylated residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-08-27 01:45:04ID requested
12016-07-06 05:21:24announced
Publication List
Rotival M, Ko JH, Srivastava PK, Kerloc'h A, Montoya A, Mauro C, Faull P, Cutillas PR, Petretto E, Behmoaras J, Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation. Mol Cell Proteomics, 14(3):484-98(2015) [pubmed]
Keyword List
curator keyword: Biomedical, Biological
submitter keyword: Macrophage, WKY, protein, rat
Contact List
Peter Allen Faull
contact affiliationMRC Clinical Sciences Centre
contact emailpeter.faull@csc.mrc.ac.uk
lab head
Peter Faull
contact affiliationMRC Clinical Sciences Centre
contact emailpeter.faull@csc.mrc.ac.uk
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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