PXD001269

PXD001269 is an original dataset announced via ProteomeXchange.

Title	Investigating macrophage multinucleation by LC-MS/MS
Description	Macrophage multinucleation (MM) is essential for various biological processes such as osteoclast-mediated bone resorption and multinucleated giant cell-associated inflammatory reactions. Here we study the molecular pathways underlying multinucleation in the rat through an integrative approach combining MS-based quantitative phosphoproteomics and transcriptome (high throughput RNA-sequencing) to identify new regulators of MM. We show that a strong metabolic shift towards HIF1-mediated glycolysis occurs at transcriptomic level during MM, together with modifications in phosphorylation of over 50 proteins including several ARF GTPase activators and polyphosphate inositol phosphatases. These results will provide a new framework for the combined analysis of transcriptional and post-translational changes during macrophage multinucleation, prioritizing essential genes and revealing the sequential events leading to the multinucleation of macrophages.
HostingRepository	PRIDE
AnnounceDate	2016-07-06
AnnouncementXML	Submission_2016-07-06_05:21:22.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001269
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Peter Faull
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; dehydrated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-27 01:45:04	ID requested
⏵ 1	2016-07-06 05:21:24	announced

Rotival M, Ko JH, Srivastava PK, Kerloc'h A, Montoya A, Mauro C, Faull P, Cutillas PR, Petretto E, Behmoaras J, Integrating phosphoproteome and transcriptome reveals new determinants of macrophage multinucleation. Mol Cell Proteomics, 14(3):484-98(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Macrophage, WKY, protein, rat

Peter Allen Faull
contact affiliation	MRC Clinical Sciences Centre
contact email	peter.faull@csc.mrc.ac.uk
lab head
Peter Faull
contact affiliation	MRC Clinical Sciences Centre
contact email	peter.faull@csc.mrc.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD001269

PRIDE project URI

• PRIDE
  1. PXD001269
     1. Label: PRIDE project
     2. Name: Investigating macrophage multinucleation by LC-MS/MS