PXD001266 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803 |
Description | To improve ethanol production directly from CO2 in photosynthetic cyanobacterial systems, one key issue that needs to be addressed is the low ethanol tolerance of cyanobacterial cells. Our previous proteomic and transcriptomic analyses found that several regulatory proteins were up regulated by exogenous ethanol in Synechocystis sp. PCC 6803. In this study, through tolerance analysis of the gene disruption mutants of the up-regulated regulatory genes, we uncovered that one transcriptional regulator, Sll0794, was related directly to ethanol tolerance in Synechocystis. Using a quantitative iTRAQ-LC-MS/MS proteomics approach coupled with quantitative real-time reverse transcription-PCR (RT-qPCR), we further determined the possible regulatory network of Sll0794. The proteomic analysis showed that in the ∆sll0794 mutant grown under ethanol stress a total of 54 and 87 unique proteins were down- and up-regulated, respectively. In addition, electrophoretic mobility shift assays (EMSAs) demonstrated that the Sll0794 transcriptional regulator was able to bind directly to the upstream regions of sll1514, slr1512 and slr1838, which encode a 16.6 kDa small heat shock protein, a putative sodium-dependent bicarbonate transporter and a carbon dioxide concentrating mechanism protein CcmK, respectively. The study provided a proteomic description of the putative ethanol-tolerance network regulated by the sll0794 gene, and revealed new insights on the ethanol-tolerance regulatory mechanism in Synechocystis. As the first regulatory protein discovered related to ethanol tolerance, the gene may serve as a valuable target for transcription machinery engineering to further improve ethanol tolerance in Synechocystis. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-26 |
AnnouncementXML | Submission_2014-09-26_07:11:51.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Guangsheng Pei |
SpeciesList | scientific name: Synechocystis sp. PCC 6803; NCBI TaxID: 1148; |
ModificationList | iTRAQ8plex-116 reporter+balance reagent acylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-26 02:05:16 | ID requested | |
⏵ 1 | 2014-09-26 07:11:52 | announced | |
Publication List
Song Z, Chen L, Wang J, Lu Y, Jiang W, Zhang W, A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803. Mol Cell Proteomics, 13(12):3519-32(2014) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Transcriptional regulator |
Ethanol |
Tolerance |
Synechocystis |
Contact List
Weiwen Zhang |
contact affiliation | Laboratory of Synthetic Microbiology School of Chemical Engineering & Technology Tianjin University, Tianjin 300072, P. R. China |
contact email | wwzhang8@tju.edu.cn |
lab head | |
Guangsheng Pei |
contact affiliation | Tianjin university |
contact email | peiguangsheng@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001266
- Label: PRIDE project
- Name: A transcriptional regulator Sll0794 regulates tolerance to biofuel ethanol in photosynthetic Synechocystis sp. PCC 6803