PXD001264 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Bacterial phosphoproteome |
Description | Protein phosphorylation is a crucial post-translational modification in bacteria, but has not been extensively studied because of the technical difficulty of phosphopeptide enrichment. We present a new enrichment protocol, approximately 10 times more efficient than conventional approaches in E. coli. This protocol also performed well in B. subtilis and K. pneumoniae, in terms of high coverage and phosphopeptide identification numbers. Moreover, three high-confidence Ser/Thr phosphorylation motifs as well as 29 other motifs at various confidence levels were discovered for the first time, implying that both the position of phospho-acceptor residues and the surrounding sequences are critical for kinase-substrate specificity. As for N-terminal phosphorylation, a low rate of co-occurrence of N-terminal acetylation and acidic residues at the antepenultimate position appears to be prokaryote-specific. The comprehensive prokaryotic phosphoproteomes generated by our new protocol suggest the existence of distinct phosphorylation preferences between prokaryotes and eukaryotes. |
HostingRepository | PRIDE |
AnnounceDate | 2015-09-22 |
AnnouncementXML | Submission_2015-09-22_06:46:03.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Miao-Hsia Lin |
SpeciesList | scientific name: Escherichia coli; NCBI TaxID: 562; |
ModificationList | phosphorylated residue |
Instrument | TripleTOF 5600 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-26 01:45:36 | ID requested | |
⏵ 1 | 2015-09-22 06:46:04 | announced | |
Publication List
Lin MH, Sugiyama N, Ishihama Y, Systematic profiling of the bacterial phosphoproteome reveals bacterium-specific features of phosphorylation. Sci Signal, 8(394):rs10(2015) [pubmed] |
Keyword List
curator keyword: Biological, Technical |
submitter keyword: Protein phosphorylation, LC-MS/MS |
Contact List
Miao-Hsia Lin |
contact affiliation | De[artment of Molecular and Cellular Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University |
contact email | miaohsialin@gmail.com |
lab head | |
Miao-Hsia Lin |
contact affiliation | Department of Molecular and Cellular Bioanalysis, Graduate School of Pharmaceutical Sciences, Kyoto University, Japan |
contact email | miaohsialin@gmail.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001264
- Label: PRIDE project
- Name: Bacterial phosphoproteome