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PXD001256

PXD001256 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDiethylation .
DescriptionIn this study we compared diethylation using acetaldehyde-13C2/13C1 and acetaldehyde-2H4/1H4. Light and heavy labeled samples were combined in a ratio of 1:1 and analyzed on a LC-MSMS system using an OrbitrapXL. We analyzed ten techical replicates of each labeling version (13C/12C and 2H/1H) and compared them in regards of labeling yield, quantification accuracy and precision.
HostingRepositoryPRIDE
AnnounceDate2024-10-03
AnnouncementXMLSubmission_2024-10-03_07:58:31.146.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterChristian Koehler
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListacetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-08-22 03:54:14ID requested
12024-10-03 07:58:31announced
Publication List
10.1002/rcm.7170;
Koehler CJ, Arntzen M Ø, Thiede B, The impact of carbon-13 and deuterium on relative quantification of proteins using stable isotope diethyl labeling. Rapid Commun Mass Spectrom, 29(9):830-6(2015) [pubmed]
Keyword List
curator keyword: Technical
submitter keyword: Diethylation, retention time shift, stable isotope labeling, reductive amination, OrbitrapXL
Contact List
Dr. Bernd Thiede
contact affiliationUniversity of Oslo, The Biotechnology Centre of Oslo, Norway
contact emailbernd.thiede@biotek.uio.no
lab head
Christian Koehler
contact affiliationUiO
contact emailchristian.koehler@biotek.uio.no
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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