PXD001252

PXD001252 is an original dataset announced via ProteomeXchange.

Title	Arabidopsis thaliana proteomics/phosphoproteomics
Description	Mitogen-activated protein kinases (MAPKs) target a variety of protein substrates to regulate cellular signaling processes in eukaryotes. In plants, the number of identified MAPK substrates that control plant defense responses is still limited. Here, we generated transgenic Arabidopsis thaliana plants with an inducible system to simulate in vivo activation of two stress-activated MAPKs, MPK3 and MPK6. Metabolome analysis revealed that this artificial MPK3/6 activation (without any exposure to pathogens or other stresses) is sufficient to drive the production of major defense-related metabolites, including various camalexin, indole glucosinolate and agmatine derivatives. An accompanying (phospho)proteome analysis led to detection of hundreds of potential phosphoproteins downstream of MPK3/6 activation. Besides known MAPK substrates, many candidates on this list possess typical MAPK-targeted phosphosites and in many cases, the corresponding phosphopeptides were detected by mass spectrometry. Notably, several of these putative phosphoproteins have been reported to be associated with the biosynthesis of antimicrobial defense substances (e.g. WRKY transcription factors and proteins encoded by the genes from the “PEN” pathway required for penetration resistance to filamentous pathogens). Thus, this work provides an inventory of candidate phosphoproteins, including putative direct MAPK substrates, for future analysis of MAPK-mediated defense control.
HostingRepository	PRIDE
AnnounceDate	2014-10-20
AnnouncementXML	Submission_2016-05-23_06:14:54.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001252
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ines Lassowskat
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-20 03:41:20	ID requested
1	2014-10-20 10:25:39	announced
⏵ 2	2016-05-23 06:14:55	announced	Updated publication reference for PubMed record(s): 25368622.

Lassowskat I, B, ö, ttcher C, Eschen-Lippold L, Scheel D, Lee J, Sustained mitogen-activated protein kinase activation reprograms defense metabolism and phosphoprotein profile in Arabidopsis thaliana. Front Plant Sci, 5():554(2014) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis thaliana, leaf, phosphorylation, LC-MS/MS, MAPK

Justin Lee
contact affiliation	IPB Department SEB Weinberg 3 06120 Halle
contact email	justin.lee@ipb-halle.de
lab head
Ines Lassowskat
contact affiliation	SEB
contact email	ines.lassowskat@ipb-halle.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001252
     1. Label: PRIDE project
     2. Name: Arabidopsis thaliana proteomics/phosphoproteomics