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PXD001248

PXD001248 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleDetermination of Supplier-To-Supplier and Lot-To-Lot Variability in Glycation of Recombinant Human Serum Albumin Expressed in Oryza sativa
DescriptionIn studies presented here, recombinant human serum albumins (rHSA) produced in Oryza sativa (Asian rice) (OsrHSA) from a number of suppliers have been extensively characterized and compared to plasma-derived HSA (pHSA) and rHSA expressed in yeast (Pichia pastoris and Saccharomyces cerevisiae). Modifications of the samples were identified by liquid chromatography-mass spectrometry (LC-MS) with samples further assessed with size exclusion chromatography (SEC), reversed-phase high-performance liquid chromatography (RP-HPLC), capillary electrophoresis (CE) as well as additionally biophysical techniques. LC-MS analysis identified a greater number of hexose-glycated arginine and lysine residues on OsrHSA compared to pHSA or rHSA expressed in yeast. Lot-to-lot variability of OsrHSA glycation from one manufactures supplied products were further identified. The extensive glycation of OsrHSA from multiple suppliers may have further implications for the use of OsrHSA as a therapeutic product.
HostingRepositoryPRIDE
AnnounceDate2014-09-12
AnnouncementXMLSubmission_2015-04-01_05:53:56.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001248
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterMarybeth Creskey
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListOxidation; Hex; Carbamidomethyl
InstrumentSynapt MS
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-08-18 07:06:04ID requested
12014-09-12 06:27:12announced
22015-04-01 05:53:57announcedUpdated publication reference for PubMed record(s): 25299339.
Publication List
Keyword List
curator keyword: Biomedical, Technical
submitter keyword: Albumin, HSA, Recombinant human serum albumin, Oryza sativa, Glycation
Contact List
Michael J.W. Johnston
contact affiliationCentre for Biologics Evaluation Biologics and Genetic Therapies Directorate Health Canada
contact emailmichael.johnston@hc-sc.gc.ca
lab head
Marybeth Creskey
contact affiliationHealth Canada
contact emailmarybeth.creskey@hc-sc.gc.ca
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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