PXD001248 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Determination of Supplier-To-Supplier and Lot-To-Lot Variability in Glycation of Recombinant Human Serum Albumin Expressed in Oryza sativa |
Description | In studies presented here, recombinant human serum albumins (rHSA) produced in Oryza sativa (Asian rice) (OsrHSA) from a number of suppliers have been extensively characterized and compared to plasma-derived HSA (pHSA) and rHSA expressed in yeast (Pichia pastoris and Saccharomyces cerevisiae). Modifications of the samples were identified by liquid chromatography-mass spectrometry (LC-MS) with samples further assessed with size exclusion chromatography (SEC), reversed-phase high-performance liquid chromatography (RP-HPLC), capillary electrophoresis (CE) as well as additionally biophysical techniques. LC-MS analysis identified a greater number of hexose-glycated arginine and lysine residues on OsrHSA compared to pHSA or rHSA expressed in yeast. Lot-to-lot variability of OsrHSA glycation from one manufactures supplied products were further identified. The extensive glycation of OsrHSA from multiple suppliers may have further implications for the use of OsrHSA as a therapeutic product. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-12 |
AnnouncementXML | Submission_2015-04-01_05:53:56.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001248 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Marybeth Creskey |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | Oxidation; Hex; Carbamidomethyl |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-08-18 07:06:04 | ID requested | |
1 | 2014-09-12 06:27:12 | announced | |
⏵ 2 | 2015-04-01 05:53:57 | announced | Updated publication reference for PubMed record(s): 25299339. |
Publication List
Keyword List
curator keyword: Biomedical, Technical |
submitter keyword: Albumin, HSA, Recombinant human serum albumin, Oryza sativa, Glycation |
Contact List
Michael J.W. Johnston |
contact affiliation | Centre for Biologics Evaluation Biologics and Genetic Therapies Directorate Health Canada |
contact email | michael.johnston@hc-sc.gc.ca |
lab head | |
Marybeth Creskey |
contact affiliation | Health Canada |
contact email | marybeth.creskey@hc-sc.gc.ca |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001248
- Label: PRIDE project
- Name: Determination of Supplier-To-Supplier and Lot-To-Lot Variability in Glycation of Recombinant Human Serum Albumin Expressed in Oryza sativa