PXD001247

PXD001247 is an original dataset announced via ProteomeXchange.

Title	The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages
Description	Kupffer cells are the first line of defense in the liver against pathogens, yet several microbes successfully target the liver, bypass immune surveillance, and effectively develop in this tissue. Our current, albeit poor, understanding of Kupffer cell-pathogen interactions has been largely achieved through the study of primary cells, requiring isolation from a large numbers of animals. To facilitate the study of Kupffer cell biology, an immortalized rat Kupffer cell line, RKC1, was developed. We performed a comparative global proteomic analysis of RKC1 and primary rat Kupffer cells (PRKC) to characterize their respective responses to lipopolysaccharide (LPS)-mediated immune stimulation. We identified patent differences in the proteomic response profile of RKC1 and PRKC to LPS. We observed that PRKC upregulated more immune function pathways and exhibited marked changes in cellular morphology following stimulation. We consequently analyzed the cytoskeletal signaling pathways of these cells in light of the fact that macrophages are known to induce cytoskeletal changes in response to pathogens. Our findings suggest that Kupffer cells respond differently to inflammatory stimulus than do monocyte-derived macrophages, and such data may provide insight into how pathogens, such as the malaria parasite, may have evolved mechanisms of liver entry through Kupffer cells without detection.
HostingRepository	PRIDE
AnnounceDate	2015-07-03
AnnouncementXML	Submission_2015-07-03_12:53:20.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001247
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Dingyin Tao
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	Oxidation; Carbamidomethyl
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-18 02:42:23	ID requested
⏵ 1	2015-07-03 12:53:21	announced

Tweedell R, Tao D, Dinglasan RR, The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages. Proteomics, 15(2-3):545-53(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Cytoskeleton

Kupffer cell

Macrophage

Malaria

Murine

Rhoel R. Dinglasan
contact affiliation	W. Harry Feinstone Department of Molecular Microbiology & Immunology and the Malaria Research Institute, Johns Hopkins Bloomberg School of Public Health
contact email	rdinglasan@jhu.edu
lab head
Dingyin Tao
contact affiliation	W. Harry Feinstone Department of Molecular Microbiology & Immunology
contact email	dtao@jhsph.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001247
     1. Label: PRIDE project
     2. Name: The cellular and proteomic response of primary and immortalized murine Kupffer cells following immune stimulation diverges from that of monocyte-derived macrophages