PXD001241

PXD001241 is an original dataset announced via ProteomeXchange.

Title	ERLIC-based strategies for combined quantitative proteome and phosphoproteome analysis with limited sample amounts
Description	The study aims the development of a sensitive strategy for combined quantitative proteomics and phosphoproteomics. 3 ERLIC-based strategies, applicable for low-sample-amount experiments (~150 µg per condition), were evaluated and compared . Three samle sets have been uploaded. 16 fractions ERLIC-1-SCX/RP (2 replicates) for phosphopeptide enrichment, 8 fractions SCX-EL (2 replicates), 12 fractions ERLIC-2 (2 replicates). The following 3 strategies were compared: A 2D strategy -> sample set ERLIC-1-SCX/RP. A pseudo-3Dstrategy -> sample sets ERLIC-1-SCX/RP + SCX-EL and a 3D strategy -> sample sets ERLIC-1-SCX/RP + ERLIC-2
HostingRepository	PRIDE
AnnounceDate	2015-02-25
AnnouncementXML	Submission_2015-02-25_01:02:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Loroch
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue; phosphorylated residue; 6x(13)C labeled residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-18 01:03:45	ID requested
⏵ 1	2015-02-25 01:02:47	announced

Loroch S, Schommartz T, Brune W, Zahedi RP, Sickmann A, Multidimensional electrostatic repulsion-hydrophilic interaction chromatography (ERLIC) for quantitative analysis of the proteome and phosphoproteome in clinical and biomedical research. Biochim Biophys Acta, 1854(5):460-8(2015) [pubmed]

curator keyword: Technical

submitter keyword: ERLIC, protein phosphorylation, quantitative proteomics, multidimensional separation

Albert Sickmann
contact affiliation	Leibniz-Institut für Analytische Wissenschaften - ISAS - e.V. Otto-Hahn-Straße 6b 44227 Dortmund, Germany
contact email	sickmann@isas.de
lab head
Stefan Loroch
contact affiliation	Leibniz-Institut für Analytische Wissenschaften, Otto-Hahn-Straße 6b, 44227 Dortmund
contact email	stefan.loroch@isas.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/02/PXD001241

PRIDE project URI

• PRIDE
  1. PXD001241
     1. Label: PRIDE project
     2. Name: ERLIC-based strategies for combined quantitative proteome and phosphoproteome analysis with limited sample amounts