PXD001230

PXD001230 is an original dataset announced via ProteomeXchange.

Title	Effects of EGFR, EGFRvIII, PTEN on invasion-promoting proteins secreted by glioblastoma cells (Sangar et al. 2014)
Description	In this project, we investigated the influence of EGFR pathway alterations on the 65 invasion promoting proteins in GBM secretome. We measured the rationally selected proteins through selected reaction monitoring (SRM) targeted mass spectrometry using stable isotope labeled internal peptide standards to quantity proteins in the secretome from five GBM (U87) isogenic cell lines in which EGFR, EGFRvIII and/or PTEN are expressed in cell lines.
HostingRepository	PeptideAtlas
AnnounceDate	2014-08-14
AnnouncementXML	Submission_2014-08-18_09:24:26.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Vineet Sangar
SpeciesList	scientific name: Homo sapiens; NCBI TaxID: 9606;
ModificationList	6x(13)C,4x(15)N labeled L-arginine; 6x(13)C,2x(15)N labeled L-lysine; S-carboxamidomethyl-L-cysteine
Instrument	6490 Triple Quadrupole LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-14 13:42:35	ID requested
⏵ 1	2014-08-18 09:24:27	announced

Sangar V, Funk CC, Kusebauch U, Campbell DS, Moritz RL, Price ND, Quantitative proteomic analysis reveals effects of epidermal growth factor receptor (EGFR) on invasion-promoting proteins secreted by glioblastoma cells. Mol Cell Proteomics, 13(10):2618-31(2014) [pubmed]

curator keyword: selected reaction monitoring, SRM, targeted

Vineet Sangar
contact affiliation	Institute for Systems Biology
contact email	vsangar@systemsbiology.org
dataset submitter
Dr Nathan D Price
contact affiliation	Institute for Systems Biology
contact email	nprice@systemsbiology.org
lab head

PeptideAtlas dataset URI

PASSEL experiment URI

PASSEL transition group browser URI