The workflow for targeted proteome survey of proteins encoded by chromosome 18 was performed in three steps. SRM scouting enabled to acquire the preliminary data about the presence of proteotypic peptides in the depleted plasma (HPL) and HepG2 cells (HG2). For the observed signals from target peptides the developed acquisition and qualitative analysis methods were checked and confirmed by comparison of purified non-labeled analogues for the endogenous peptides. The scheduled MRM methods were developed from the confirmed MRM methods for distinct peptides and the final data were acquired from biosamples into this dataset named "bio".