PXD001221

PXD001221 is an original dataset announced via ProteomeXchange.

Title	Targeting of non-phosphorylated lysosomal enzymes involves secretion and Lrp1-mediated recapture
Description	Most lysosomal enzymes require mannose 6-phosphate (M6P) residues for efficient receptor-mediated lysosomal targeting. Although the lack of M6P results in missorting and hypersecretion, selected lysosomal enzymes reach normal levels in lysosomes of various cell types suggesting the existence of M6P-independent transport routes. SILAC-based analysis of purified lysosomes from M6P-deficient mouse fibroblasts (PTki) revealed unchanged amounts of 11 lysosomal enzymes, including cathepsin D and B (CtsD, CtsB), while demonstrating mossiorting of a variety of soluble lysosomal enzymes
HostingRepository	PRIDE
AnnounceDate	2015-05-01
AnnouncementXML	Submission_2015-05-01_09:06:10.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Thelen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	6x(13)C: 4x(15)N labeled L-arginine; monohydroxylated residue; acetylated residue; 6x(13)C: 2x(15)N labeled L-lysine
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-13 02:51:13	ID requested
⏵ 1	2015-05-01 09:06:12	announced

Markmann S, Thelen M, Cornils K, Schweizer M, Brocke-Ahmadinejad N, Willnow T, Heeren J, Gieselmann V, Braulke T, Kollmann K, Lrp1/LDL Receptor Play Critical Roles in Mannose 6-Phosphate-Independent Lysosomal Enzyme Targeting. Traffic, 16(7):743-59(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: low density lipoprotein-related receptor/ lysosomal targeting/ mannose 6-phosphate/ receptor-mediated endocytosis/ sortilin

Prof. Dr. Volkmar Gieselmann
contact affiliation	University Bonn Institute for Biochemistry and Molecular Biology Nussallee 11 D-53115 Bonn
contact email	gieselmann@ibmb.uni-bonn.de
lab head
Melanie Thelen
contact affiliation	Institute for Biochemistry and Molecular Biology, University Bonn, Germany
contact email	mthelen@uni-bonn.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/05/PXD001221

PRIDE project URI

• PRIDE
  1. PXD001221
     1. Label: PRIDE project
     2. Name: Targeting of non-phosphorylated lysosomal enzymes involves secretion and Lrp1-mediated recapture