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PXD001214

PXD001214 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleRsp5/Nedd4 is the major ubiquitin ligase that targets cytosolic misfolded proteins upon heat-stress
DescriptionThe heat-shock response is a complex cellular program that induces major changes in protein translation, folding and degradation to alleviate toxicity caused by protein misfolding. While heat-shock has been widely used to study proteostasis, it remained unclear how misfolded proteins are targeted for proteolysis in these conditions. We found that Rsp5 and its mammalian homologue Nedd4 are the main E3-ligases responsible for the increased ubiquitination induced by heat-stress. We determined that Rsp5 ubiquitinates cytosolic misfolded proteins upon heat-shock for proteasome degradation. We found that ubiquitination of heat-induced substrates requires the Hsp40 co-chaperone Ydj1 that is further associated with Rsp5 upon heat-shock. Additionally, ubiquitination is also promoted by PY Rsp5-binding motifs found primarily in the structured regions of stress-induced substrates, which can act as heat-induced degrons. Our results support a bipartite recognition mechanism combining direct and chaperone-dependent ubiquitination of misfolded cytosolic proteins by Rsp5.
HostingRepositoryPRIDE
AnnounceDate2024-10-22
AnnouncementXMLSubmission_2024-10-22_04:03:50.663.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterNeng Fang
SpeciesList scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationListcarbamoylated residue; 6x(13)C; ubiquitination signature dipeptidyl lysine; phosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue; IMID d4
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-08-08 03:26:16ID requested
12016-07-06 06:04:30announced
22024-10-22 04:03:56announced2024-10-22: Updated project metadata.
Publication List
Fang NN, Chan GT, Zhu M, Comyn SA, Persaud A, Deshaies RJ, Rotin D, Gsponer J, Mayor T, Rsp5/Nedd4 is the main ubiquitin ligase that targets cytosolic misfolded proteins following heat stress. Nat Cell Biol, 16(12):1227-37(2014) [pubmed]
10.1038/ncb3054;
Keyword List
curator keyword: Biological
submitter keyword: Rsp5, heat-shock, cytosolic protein quality control
Contact List
Nancy Fang
contact affiliationMayor Lab the Centre for High-Thoughput Biology Dept. of Biochemistry and Molecular Biology the University of British Columbia
contact emailnancynfang@gmail.com
lab head
Neng Fang
contact affiliationCentre for High Throughput Biology
contact emailnengf@interchange.ubc.ca
dataset submitter
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Dataset FTP location
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