PXD001204

PXD001204 is an original dataset announced via ProteomeXchange.

Title	Multiple Proteases to Localize Oxidation Sites
Description	Proteins present in cellular environments with high levels of reactive oxygen and nitrogen species and/or low levels of antioxidants are highly susceptible to oxidative post-translational modification (PTM). Irreversible oxidative PTMs can generate a complex distribution of modified protein molecules, recently termed as proteoforms. Using ubiquitin as a model system, we mapped oxidative modification sites using trypsin, Lys-C, and Glu-C peptides. Several M+16 Da proteoforms were detected as well as proteoforms that include other previously unidentified oxidative modifications. This work highlights the use of multiple protease digestions to give insights to the complexity of oxidative modifications possible in bottom-up analyses.
HostingRepository	PRIDE
AnnounceDate	2016-07-06
AnnouncementXML	Submission_2016-07-06_12:29:27.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001204
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Liqing Gu
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-06 01:45:49	ID requested
⏵ 1	2016-07-06 12:29:28	announced

Gu L, Robinson RA, Multiple proteases to localize oxidation sites. PLoS One, 10(3):e0116606(2015) [pubmed]

curator keyword: Biological, Technical

submitter keyword: oxidation, ubiquitin, top-down, bottom-up proteomics, Orbitrap mass spectrometry

Rena A. S. Robinson
contact affiliation	University of Pittsburgh
contact email	rena@pitt.edu
lab head
Liqing Gu
contact affiliation	University of Pittsburgh
contact email	lig32@pitt.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD001204

PRIDE project URI

• PRIDE
  1. PXD001204
     1. Label: PRIDE project
     2. Name: Multiple Proteases to Localize Oxidation Sites