PXD001202

PXD001202 is an original dataset announced via ProteomeXchange.

Title	Identification of cytoplasmic proteins interacting with unliganded estrogen receptor α and β- in human breast cancer cells
Description	Estrogen Receptor subtypes (ERα and ERβ) are transcription factors sharing similar structure, however, they often perform opposite roles in breast cancer’s cell proliferation and tumor progression. Besides the well-characterized genomic actions of ERs upon ligand binding, rapid non-genomic cytoplasmic changes together with the recently discovered ligand-free action of ERs are emerging as key regulators of tumorigenesis. The identification of cytoplasmic interaction partners of unliganded ERα and ERβ may help characterize the molecular basis of the extra-nuclear mechanism of action of these receptors, revealing novel mechanisms to explain their role in breast cancer response or resistance to endocrine therapy. To this aim, in this study, cytoplasmic extracts from stably expressing TAP-ERα and -ERβ MCF-7 cell clones were subjected to interaction proteomics in the absence of estrogen stimulation, leading to the identification of 84 and 142 proteins associated with unliganded ERα and ERβ, respectively. Functional analyses of ER subtype-specific interactomes revealed significant differences in the molecular pathways associated to each receptor in the cytoplasm. This work reports the first identification of the unliganded ERα and ERβ cytoplasmic interactomes in breast cancer cells, providing novel experimental evidence on the non-genomic effects of ERs in the absence of hormonal stimulus.
HostingRepository	PRIDE
AnnounceDate	2015-03-11
AnnouncementXML	Submission_2015-03-11_06:46:04.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Tuula Nyman
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-05 07:14:06	ID requested
⏵ 1	2015-03-11 06:46:05	announced

Stellato C, Nassa G, Tarallo R, Giurato G, Ravo M, Rizzo F, Marchese G, Alexandrova E, Cordella A, Baumann M, Nyman TA, Weisz A, Ambrosino C, in human breast cancer cells. Proteomics, 15(11):1801-7(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: estrogen receptor, TAP-tag, LC-MS/MS

Tuula Nyman
contact affiliation	Institute of Biotechnology, University of Helsinki
contact email	tuula.nyman@helsinki.fi
lab head
Tuula Nyman
contact affiliation	Institute of Biotechnology
contact email	tuula.nyman@helsinki.fi
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001202
     1. Label: PRIDE project
     2. Name: Identification of cytoplasmic proteins interacting with unliganded estrogen receptor α and β- in human breast cancer cells