PXD001195

PXD001195 is an original dataset announced via ProteomeXchange.

Title	Dynamics of AMPAR proteome across brain regions and in postnatal development
Description	Native AMPA receptors (AMPAR) in the mammalian brain are macromolecular complexes whose functional characteristics vary across brain regions and postnatal development and change in response to neuronal activity. Both, structural and functional properties of the AMPARs are determined by their proteome, the ensemble of their protein building blocks. Here we use high-resolution quantitative mass spectrometry to analyze the entire pool of AMPARs affinity-isolated from distinct brain regions, selected sets of synapses/neurons and from whole brains at distinct stages of postnatal development. These analyses show that the AMPAR proteome is largely dynamic in both space and time: AMPARs exhibited profound region-specificity in their architecture and the constituents building their core and periphery. Likewise, AMPARs (ex)change numerous of their building blocks during postnatal development. These results provide an as yet unique resource for analysis of the individual subunits of native AMPAR complexes and their role in excitatory neurotransmission.
HostingRepository	PRIDE
AnnounceDate	2016-07-08
AnnouncementXML	Submission_2016-07-08_08:10:18.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001195
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Alexander Haupt
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	Oxidation; Phospho; Gln->pyro-Glu; Propionamide; Glu->pyro-Glu; Carbamidomethyl; Deamidated
Instrument	LTQ Orbitrap; LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-08-04 06:34:19	ID requested
1	2016-07-06 14:02:01	announced
⏵ 2	2016-07-08 08:10:19	announced	Updated project metadata.

Schwenk J, Baehrens D, Haupt A, Bildl W, Boudkkazi S, Roeper J, Fakler B, Schulte U, Regional diversity and developmental dynamics of the AMPA-receptor proteome in the mammalian brain. Neuron, 84(1):41-54(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: rat, brain regions, AMPA receptor, proteome, development, LC-MSMS, AP-MS

Bernd Fakler
contact affiliation	Institute of Physiology II, University of Freiburg, Germany
contact email	bernd.fakler@physiologie.uni-freiburg.de
lab head
Alexander Haupt
contact affiliation	University of Freiburg, Germany
contact email	alexander.haupt@physiologie.uni-freiburg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001195
     1. Label: PRIDE project
     2. Name: Dynamics of AMPAR proteome across brain regions and in postnatal development