PXD001190

PXD001190 is an original dataset announced via ProteomeXchange.

Title	Similar is not the same: Differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi
Description	The transcriptional activator XlnR (Xlr1/Xyr1) is a major regulator in fungal xylan and cellulose degradation as well as in the utilization of d-xylose via the pentose catabolic pathway. XlnR homologs are commonly found in filamentous ascomycetes and often assumed to have the same function in different fungi. However, a comparison of the saprobe Aspergillus niger and the plant pathogen Magnaporthe oryzae showed different phenotypes for deletion strains of XlnR. In this study wild type and xlnR/xlr1/xyr1 mutants of five fungi were compared: Fusarium graminearum, M. oryzae, Trichoderma reesei, A. niger and Aspergillus nidulans. Growth profiling on relevant substrates and a detailed analysis of the secretome as well as extracellular enzyme activities demonstrated a common role of this regulator in activating genes encoding the main xylanolytic enzymes. However, large differences were found in the set of genes that is controlled by XlnR in the different species, resulting in the production of different extracellular enzyme spectra by these fungi. This comparison emphasizes the functional diversity of a fine-tuned (hemi-)cellulolytic regulatory system in filamentous fungi, which might be related to the adaptation of fungi to their specific biotopes.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_05:00:55.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Harm Post
SpeciesList	scientific name: Aspergillus nidulans TN02A3; NCBI TaxID: 1263753; scientific name: Trichoderma reesei QM9414; NCBI TaxID: 334564; scientific name: Aspergillus niger; NCBI TaxID: 5061; scientific name: Fusarium graminearum GZ3639; NCBI TaxID: 449239; scientific name: Magnaporthe oryzae B157; NCBI TaxID: 1348879;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-31 02:54:09	ID requested
1	2014-08-01 02:42:53	announced
⏵ 2	2014-10-23 05:00:56	announced	Updated project metadata.

Klaubauf S, Narang HM, Post H, Zhou M, Brunner K, Mach-Aigner AR, Mach RL, Heck AJR, Altelaar AFM, de Vries RP, Similar is not the same: differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi. Fungal Genet Biol, 72():73-81(2014) [pubmed]

curator keyword: Biological

submitter keyword: XlnR, Xylanase regulator, Xylan degradation, Magnaporthe oryzae, Fusarium graminearum, Trichoderma reesei, Aspergillus

Ronald Peter de Vries
contact affiliation	1CBS-KNAW Fungal Biodiversity Centre, Utrecht, The Netherlands
contact email	r.devries@cbs.knaw.nl
lab head
Harm Post
contact affiliation	Biomolecular Mass Spectrometry and Proteomics Group
contact email	h.post@uu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001190
     1. Label: PRIDE project
     2. Name: Similar is not the same: Differences in the function of the (hemi-)cellulolytic regulator XlnR (Xlr1/Xyr1) in filamentous fungi