PXD001184

PXD001184 is an original dataset announced via ProteomeXchange.

Title	Phosphoproteomic Analysis Suggests Compensatory Effects in the Piriform Cortex Region of the VX-Exposed Rat
Description	To gain insight into the toxicity induced by VX, a mass spectrometry based phosphoproteomics approach was employed to understand the signaling modulated by VX toxicity in piriform cortex region of the rat brain. We have employed isobaric-based TMT labeling and titanium dioxide- based enrichment strategy to identify and quantify the changes that are modulated by VX. We observed a temporal association of changes in the phosphorylation status of proteins over a 24 hour time course in rats exposed to 1x LD50 VX, with the most notable changes by the first measured time point, 1 hour post exposure. These data fell into five main functional classes of proteins directly or indirectly influenced by changes in phosphorylation: 1) Ion channels/transporters, including ATPases, 2) Kinases/Phosphatases, 3) GTPases, 4) Structural related proteins, and 5) Transcriptional regulatory proteins. This study is the first quantitative phosphoproteomics analysis of VX toxicity in the brain. Understanding the toxicity and compensatory signaling mechanisms will improve the understanding of the complex toxicity of VX in the brain, and aid in the elucidation of novel molecular targets allowing for improved countermeasure development.
HostingRepository	PRIDE
AnnounceDate	2015-01-07
AnnouncementXML	Submission_2015-01-07_02:16:15.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jennifer Sekowski
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	monohydroxylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-30 01:50:08	ID requested
⏵ 1	2015-01-07 02:16:16	announced

Nirujogi RS, Wright JD, Manda SS, Zhong J, Na CH, Meyerhoff J, Benton B, Jabbour R, Willis K, Kim MS, Pandey A, Sekowski JW, Phosphoproteomic analysis reveals compensatory effects in the piriform cortex of VX nerve agent exposed rats. Proteomics, 15(2-3):487-99(2015) [pubmed]

curator keyword: Biological

submitter keyword: Phosphoproteomics, VX, signaling

Jennifer W. Sekowski
contact affiliation	US Army Edgewood Chemical Biological Center, Biosciences Division, E3150, Aberdeen Proving Ground, MD 21010
contact email	jennifer.w.sekowski.civ@mail.mil
lab head
Jennifer Sekowski
contact affiliation	US Army Edgewood Chemical Biological Center
contact email	jennifer.w.sekowski.civ@mail.mil
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001184
     1. Label: PRIDE project
     2. Name: Phosphoproteomic Analysis Suggests Compensatory Effects in the Piriform Cortex Region of the VX-Exposed Rat