PXD001181

PXD001181 is an original dataset announced via ProteomeXchange.

Title	Phosphosite-specific regulation of the oxidative-stress response of P. brasiliensis: A Shotgun Phosphoproteomic Analysis
Description	Paracoccidioides brasiliensis, a thermally dimorphic fungus, is the causative agent of paracoccidioidomycosis, a systemic mycosis that is widespread in Latin America. P. brasiliensis infection occurs when conidia or mycelial fragments are inhaled by the host, which causes these cells to transition to the yeast form. The development of disease requires conidia inside host alveoli to differentiate into yeast cells in a temperature-dependent manner. This fungus is a facultative intracellular pathogen able to survive and replicate inside non-activated macrophages. Therefore, the survival of P. brasiliensis inside the host depends on the ability to adapt to oxidative stress induced by immune cells, especially alveolar macrophages. For several years, reactive oxygen species (ROS) were only associated with pathological processes. Currently, a plethora of roles for ROS in cell signaling have emerged. We have previously reported that low ROS concentrations cause cell proliferation in the human pathogenic fungus P. brasiliensis. In the present report, we investigated the influence of phosphorylation events in that process. Using a mass spectrometry-based approach, we mapped 440 phosphorylation sites in 230 P. brasiliensis proteins and showed that phosphorylation at different sites determines fungal responses to oxidative stress. Furthermore, we described, for the first time, the presence of a two-component signal transduction system in P. brasiliensis. These findings will help us to understand the phosphorylation events involved in the oxidative stress response.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:35:01.953.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alexandre Keiji Tashima
SpeciesList	scientific name: Paracoccidioides brasiliensis (strain Pb18); NCBI TaxID: 502780;
ModificationList	phosphorylated residue; acetylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-29 01:00:28	ID requested
1	2022-03-02 17:16:36	announced
⏵ 2	2024-10-22 05:35:02	announced	2024-10-22: Updated project metadata.

10.1016/j.micinf.2016.08.004;

Chaves AF, Castilho DG, Navarro MV, Oliveira AK, Serrano SM, Tashima AK, Batista WL, Phosphosite-specific regulation of the oxidative-stress response of Paracoccidioides brasiliensis: a shotgun phosphoproteomic analysis. Microbes Infect, 19(1):34-46(2017) [pubmed]

curator keyword: Biological, Biomedical

submitter keyword: Paracoccidioides brasiliensis, post-translational modification,cell proliferation, phosphorylation, oxidative stress

Alexandre Keiji Tashima
contact affiliation	Departamento de Bioquimica, Escola Paulista de Medicina, Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
lab head
Alexandre Keiji Tashima
contact affiliation	Universidade Federal de Sao Paulo
contact email	aktashima@unifesp.br
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001181
     1. Label: PRIDE project
     2. Name: Phosphosite-specific regulation of the oxidative-stress response of P. brasiliensis: A Shotgun Phosphoproteomic Analysis