PXD001175

PXD001175 is an original dataset announced via ProteomeXchange.

Title	Proteomic dissection of nanoparticle-containing vesicles reveals a major role for a macropinocytic-like uptake mechanism and multivesicular bodies during intracellular nanoparticle trafficking
Description	Rational design of nanocarriers for drug delivery approaches requires an unbiased knowledge of uptake mechanisms and intracellular trafficking pathways. Here we dissected these processes using a quantitative proteomics approach. We isolated intracellular vesicles containing superparamagnetic iron oxide polystyrene nanoparticles and analyzed their protein composition by label free quantitative mass spectrometry. The proteomic snapshot of organelle marker proteins revealed that an atypical macropinocytic-like mechanism mediated the entry of nanoparticles. We show that the entry mechanism is controlled by actin reorganization, atypical macropinocytic signaling and ADP-ribosylation factor 1. Additionally, our proteomics data demonstrated a central role for multivesicular bodies and multilamellar lysosomes in trafficking and final nanoparticle storage. This was confirmed by confocal microscopy and cryo-TEM measurements. By quantitatively analyzing the protein composition of nanoparticle-containing vesicles, our study clearly defines the routes of nanoparticle entry, intracellular trafficking and the proteomic milieu of a nanoparticle-containing vesicle.
HostingRepository	PRIDE
AnnounceDate	2014-09-25
AnnouncementXML	Submission_2014-09-25_03:20:23.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Stefan Tenzer
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Synapt G2-S HDMS

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-25 03:00:45	ID requested
⏵ 1	2014-09-25 03:20:24	announced

Hofmann D, Tenzer S, Bannwarth MB, Messerschmidt C, Glaser SF, Schild H, Landfester K, Mail, ä, nder V, Mass spectrometry and imaging analysis of nanoparticle-containing vesicles provide a mechanistic insight into cellular trafficking. ACS Nano, 8(10):10077-88(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, HeLa, Synapt G2S, DIA, HDMSE, nanoparticles, vesicles, endosomes, intracellular trafficking

Stefan Tenzer
contact affiliation	Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany
contact email	tenzer@uni-mainz.de
lab head
Stefan Tenzer
contact affiliation	Institute for Immunology, University Medical Center of the Johannes Gutenberg University Mainz, Mainz, Germany.
contact email	tenzer@uni-mainz.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001175

PRIDE project URI

• PRIDE
  1. PXD001175
     1. Label: PRIDE project
     2. Name: Proteomic dissection of nanoparticle-containing vesicles reveals a major role for a macropinocytic-like uptake mechanism and multivesicular bodies during intracellular nanoparticle trafficking