PXD001171

PXD001171 is an original dataset announced via ProteomeXchange.

Title	Human Serum LC-MS/MS
Description	Associating changes in protein levels with the onset of cancer has proven as an effective approach to identify clinically relevant diagnostic biomarkers. In the present study, we used sera from 205 patients representing two cohorts for biomarker discovery using label-free proteomic analysis by liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS). We performed first untargeted proteomic analysis of sera to identify candidate proteins with statistically significant difference between hepatocellular carcinoma (HCC) and patients with liver cirrhosis. These proteins were later on evaluated through targeted quantitation by multiple reaction monitoring (MRM) on a triple quadrupole mass spectrometer. The results of this study illustrate the power of combining untargeted and targeted quantitation methods for a comprehensive serum proteomic analysis, to investigate changes in protein levels and discover novel diagnostic biomarkers.
HostingRepository	PRIDE
AnnounceDate	2015-06-02
AnnouncementXML	Submission_2015-06-02_06:39:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Habtom Ressom
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-24 00:33:11	ID requested
⏵ 1	2015-06-02 06:39:46	announced

Tsai TH, Song E, Zhu R, Di Poto C, Wang M, Luo Y, Varghese RS, Tadesse MG, Ziada DH, Desai CS, Shetty K, Mechref Y, Ressom HW, LC-MS/MS-based serum proteomics for identification of candidate biomarkers for hepatocellular carcinoma. Proteomics, 15(13):2369-81(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, Hepatocellular carcinoma, Liver cirrhosis, Serum, LC-MS/MS

Habtom W. Ressom
contact affiliation	Ressom Lab, Department of Oncology, Lambardi Comprehensive Cancer Center, Georgetown University Medical Center, Washington, DC, USA
contact email	hwr@georgetown.edu
lab head
Habtom Ressom
contact affiliation	Georgetown University
contact email	hwr@georgetown.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/06/PXD001171

PRIDE project URI

• PRIDE
  1. PXD001171
     1. Label: PRIDE project
     2. Name: Human Serum LC-MS/MS