PXD001163

PXD001163 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of the Plasmodium male gamete reveals a key role for glycolysis in flagellar motility
Description	Gametogenesis and fertilization play crucial roles in malaria transmission. While male gametes are thought to be amongst the simplest eukaryotic cells and are proven targets of transmission blocking immunity, little is known about their molecular organization. For example, the pathways of energy metabolism that power motility, a feature that facilitates gamete encounter and fertilization, is unknown. To gain more insight into male gamete, we performed the first proteomic analysis of purified Plasmodium berghei microgametes. We recovered 615 proteins, which include all male gamete proteins described thus far. Amongst the most abundant proteins were the 11 enzymes of the glycolytic pathway. We localized the hexose transporter to the gamete plasma membrane and show that microgamete motility can be suppressed effectively by inhibitors of this transporter, and of the glycolytic pathway. Taken together, these results suggest that glycolysis is the exclusive source of energy for microgamete motility. Considering that this proteome provides a unique platform for the understanding of the original features of the male gamete, we discuss not only the mitochondria-independent energy metabolism but also flagellar structure and intra-flagellar transport-independent assembly, another divergent feature of these cells.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_05:00:37.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ceereena Ubaida Mohien
SpeciesList	scientific name: Plasmodium sp. pBT9; NCBI TaxID: 257632;
ModificationList	iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-22 01:39:01	ID requested
1	2014-08-18 01:19:40	announced
⏵ 2	2014-10-23 05:00:38	announced	Updated project metadata.

Talman AM, Prieto JH, Marques S, Ubaida-Mohien C, Lawniczak M, Wass MN, Xu T, Frank R, Ecker A, Stanway RS, Krishna S, Sternberg MJ, Christophides GK, Graham DR, Dinglasan RR, Yates JR, Sinden RE, Proteomic analysis of the Plasmodium male gamete reveals the key role for glycolysis in flagellar motility. Malar J, 13():315(2014) [pubmed]

curator keyword: Biological

submitter keyword: Plasmodium, male gametes, gametogenesis, proteomics

Arthur M. Talman
contact affiliation	Division of Cell and Molecular Biology, Imperial College London, UK
contact email	amtalman@gmail.com
lab head
Ceereena Ubaida Mohien
contact affiliation	Johns Hopkins School of Medicine
contact email	ceereena@jhmi.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001163

PRIDE project URI

• PRIDE
  1. PXD001163
     1. Label: PRIDE project
     2. Name: Proteomic analysis of the Plasmodium male gamete reveals a key role for glycolysis in flagellar motility