PXD001158

PXD001158 is an original dataset announced via ProteomeXchange.

Title	Profiling the proteome of Mycobacterium tuberculosis during dormancy and reactivation
Description	Tuberculosis, caused by Mycobacterium tuberculosis, still remains a major global health problem. The main obstacle in eradicating this disease is the ability of this pathogen to remain dormant in macrophages, and to get reactivated later under immuno-compromised conditions. The physiology of hypoxic nonreplicating M. tuberculosis is well studied using many in vitro dormancy models. However, the physiological changes that take place during the shift from dormancy to aerobic growth (reactivation) have rarely been subjected to a detailed investigation. In this study, we developed an in vitro reactivation system by re-aerating bacteria that were made dormant employing Wayne’s dormancy model, and compared the proteome profiles of dormant and reactivated bacteria using label-free one-dimensional LC/MS/MS analysis.
HostingRepository	PRIDE
AnnounceDate	2015-06-01
AnnouncementXML	Submission_2015-06-01_02:38:17.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001158
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Ajay Kumar
SpeciesList	scientific name: Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv); NCBI TaxID: 83332;
ModificationList	Oxidation; Carbamidomethyl
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-18 05:11:55	ID requested
⏵ 1	2015-06-01 02:38:19	announced

Gopinath V, Raghunandanan S, Gomez RL, Jose L, Surendran A, Ramachandran R, Pushparajan AR, Mundayoor S, Jaleel A, Kumar RA, Profiling the Proteome of Mycobacterium tuberculosis during Dormancy and Reactivation. Mol Cell Proteomics, 14(8):2160-76(2015) [pubmed]

submitter keyword: Mycobacterium tuberculosis, dormancy, reactivation, LC-MSMS, quantitative proteomics

R. Ajay Kumar
contact affiliation	Mycobacterium Research Group, Rajiv Gandhi Centre for Biotechnology, Thycaud P.O., Thiruvananthapuram.
contact email	rakumar@rgcb.res.in
lab head
Ajay Kumar
contact affiliation	Mycobacterium Research Group,Rajiv Gandhi Centre for Biotechnology,Trivandrum
contact email	rakumar@rgcb.res.in
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001158
     1. Label: PRIDE project
     2. Name: Profiling the proteome of Mycobacterium tuberculosis during dormancy and reactivation