PXD001155

PXD001155 is an original dataset announced via ProteomeXchange.

Title	An organellar Nα-acetyltransferase Naa60 acetylates cytosolic N-termini of transmembrane proteins and maintains Golgi integrity
Description	N-terminal acetylation is a major and vital protein modification catalysed by N-terminal acetyltransferases (NATs). NatF or Nα-acetyltransferase 60 (Naa60) was recently identified as a NAT in higher eukaryotes. Here, we found that Naa60 differs from all other known NATs by its Golgi localization. A new membrane topology assay named PROMPT and a selective membrane permeabilization assay established Naa60 to face the cytosolic side of intracellular membranes. An Nt-acetylome analysis of NAA60 knockdown cells revealed that Naa60, as opposed to other NATs, specifically acetylates transmembrane proteins, and that is has a preference for N-termini facing the cytosol. Moreover, NAA60 knockdown caused Golgi fragmentation, indicating an important role in maintenance of Golgi structural integrity. This work uncovers the first NAT associated with membranous compartments and establishes N-terminal acetylation as a common modification among transmembrane proteins, a thus far poorly characterized part of the N-terminal acetylome.
HostingRepository	PRIDE
AnnounceDate	2019-06-04
AnnouncementXML	Submission_2019-06-04_07:40:45.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001155
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jonathan Vandenbussche
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	L-methionine sulfoxide; 6x(13)C; L-methionine (S)-sulfoxide; 3x(2)H labeled N6-acetyl-L-lysine; N-acetylated residue; S-carboxymethyl-L-cysteine
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-17 05:15:33	ID requested
⏵ 1	2019-06-04 07:40:46	announced

Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, St, ø, ve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T, -acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity. Cell Rep, 10(8):1362-74(2015) [pubmed]

curator keyword: Biological

submitter keyword: Naa60, N-terminal COFRADIC

Kris Gevaert
contact affiliation	VIB, department of Medical Protein Research Ugent, department of Biochemistry
contact email	kris.gevaert@vib-ugent.be
lab head
Jonathan Vandenbussche
contact affiliation	Biochemistry
contact email	jonathan.vandenbussche@vib-ugent.be
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2019/06/PXD001155

PRIDE project URI

• PRIDE
  1. PXD001155
     1. Label: PRIDE project
     2. Name: An organellar Nα-acetyltransferase Naa60 acetylates cytosolic N-termini of transmembrane proteins and maintains Golgi integrity