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PXD001155

PXD001155 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleAn organellar Nα-acetyltransferase Naa60 acetylates cytosolic N-termini of transmembrane proteins and maintains Golgi integrity
DescriptionN-terminal acetylation is a major and vital protein modification catalysed by N-terminal acetyltransferases (NATs). NatF or Nα-acetyltransferase 60 (Naa60) was recently identified as a NAT in higher eukaryotes. Here, we found that Naa60 differs from all other known NATs by its Golgi localization. A new membrane topology assay named PROMPT and a selective membrane permeabilization assay established Naa60 to face the cytosolic side of intracellular membranes. An Nt-acetylome analysis of NAA60 knockdown cells revealed that Naa60, as opposed to other NATs, specifically acetylates transmembrane proteins, and that is has a preference for N-termini facing the cytosol. Moreover, NAA60 knockdown caused Golgi fragmentation, indicating an important role in maintenance of Golgi structural integrity. This work uncovers the first NAT associated with membranous compartments and establishes N-terminal acetylation as a common modification among transmembrane proteins, a thus far poorly characterized part of the N-terminal acetylome.
HostingRepositoryPRIDE
AnnounceDate2019-06-04
AnnouncementXMLSubmission_2019-06-04_07:40:45.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001155
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterJonathan Vandenbussche
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListL-methionine sulfoxide; 6x(13)C; L-methionine (S)-sulfoxide; 3x(2)H labeled N6-acetyl-L-lysine; N-acetylated residue; S-carboxymethyl-L-cysteine
InstrumentLTQ Orbitrap
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-07-17 05:15:33ID requested
12019-06-04 07:40:46announced
Publication List
Aksnes H, Van Damme P, Goris M, Starheim KK, Marie M, St, ø, ve SI, Hoel C, Kalvik TV, Hole K, Glomnes N, Furnes C, Ljostveit S, Ziegler M, Niere M, Gevaert K, Arnesen T, -acetyltransferase, naa60, acetylates cytosolic N termini of transmembrane proteins and maintains Golgi integrity. Cell Rep, 10(8):1362-74(2015) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Naa60, N-terminal COFRADIC
Contact List
Kris Gevaert
contact affiliationVIB, department of Medical Protein Research Ugent, department of Biochemistry
contact emailkris.gevaert@vib-ugent.be
lab head
Jonathan Vandenbussche
contact affiliationBiochemistry
contact emailjonathan.vandenbussche@vib-ugent.be
dataset submitter
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Dataset FTP location
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PRIDE project URI
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