PXD001153

PXD001153 is an original dataset announced via ProteomeXchange.

Title	Quantitative Phosphoproteomics Reveals Crosstalk Between Phosphorylation and O-GlcNAc in the DNA Damage Response Pathway
Description	The modification of intracellular proteins by monosaccharides of O-linked linked β-N-acetylglucosamine (O-GlcNAc) is thought to mediate proteins by regulating protein phosphorylation, either by modifying and modulating protein kinases or by competing with phosphorylation for hydroxyl residues. To provide molecular insight into the pathways regulated by O-GlcNAc that promote survival in basal conditions or during cellular stress, we have utilized SILAC-based quantitative proteomics to carry out comparisons of site-specific phosphorylation in OGT wild-type (WT) and Null cells. Quantitation of the phosphoproteome demonstrated that out of 5,529 serine/threonine/tyrosine phosphorylated sites, 231 phosphosites were upregulated and 133 downregulated in the absence of O-GlcNAc. The data identified widespread changes in the phosphoproteome upon removal of O-GlcNAc, suggesting that O-GlcNAc regulates processes such as the cell cycle, genomic stability, inflammatory responses and lysosomal biogenesis.
HostingRepository	PRIDE
AnnounceDate	2015-01-28
AnnouncementXML	Submission_2015-01-28_02:35:16.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Akhilesh Pandey
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue; phosphorylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-16 01:25:32	ID requested
⏵ 1	2015-01-28 02:35:17	announced

Zhong J, Martinez M, Sengupta S, Lee A, Wu X, Chaerkady R, Chatterjee A, O'Meally RN, Cole RN, Pandey A, Zachara NE, Quantitative phosphoproteomics reveals crosstalk between phosphorylation and O-GlcNAc in the DNA damage response pathway. Proteomics, 15(2-3):591-607(2015) [pubmed]

curator keyword: Biological

submitter keyword: Mouse embryonic fibroblast, OGT knockout, ATM

Akhilesh Pandey
contact affiliation	McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University, Baltimore, Maryland 21205 USA
contact email	pandey@jhmi.edu
lab head
Akhilesh Pandey
contact affiliation	Johns Hopkins University
contact email	pandey@jhmi.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001153
     1. Label: PRIDE project
     2. Name: Quantitative Phosphoproteomics Reveals Crosstalk Between Phosphorylation and O-GlcNAc in the DNA Damage Response Pathway