PXD001135

PXD001135 is an original dataset announced via ProteomeXchange.

Title	Relative protein quantification in mouse hippocampal synaptosomes during aging series
Description	Age-related cognitive decline is a serious health concern in our aging society. Decreased cognitive function observed during healthy brain aging is most likely caused by changes in brain connectivity and synaptic dysfunction in particular brain regions. Here we show that aged C57BL/6J wildtype mice have hippocampus-dependent spatial memory impairments. To identify the molecular mechanisms that are relevant to these memory deficits we investigated the temporal profile of mouse hippocampal synaptic proteome changes at 20, 40, 50, 60, 70, 80, 90 and 100 weeks of age. Extracellular matrix proteins were the only group of proteins that showed a robust and progressive upregulation over time. This was confirmed by immunoblotting and histochemical analysis, indicating that the increased levels of hippocampal extracellular matrix may limit synaptic plasticity as a potential cause of age-related cognitive decline. In addition, we observed that stochasticity in synaptic protein expression increased with age, in particular for proteins that were previously linked with various neurodegenerative diseases, whereas low variance in expression was observed for proteins that play a basal role in neuronal function and synaptic neurotransmission. Together, our findings show that both specific changes and increased variance in synaptic protein expression are associated with aging and may underlie reduced synaptic plasticity and impaired cognitive performance at old age.
HostingRepository	PRIDE
AnnounceDate	2014-09-10
AnnouncementXML	Submission_2014-09-10_04:19:56.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001135
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Pim van Nierop
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iTRAQ8plex-116 reporter+balance reagent acylated residue; methylthiolated residue
Instrument	TOF/TOF 5800

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-15 02:53:55	ID requested
⏵ 1	2014-09-10 04:19:57	announced

V, é, gh MJ, Rausell A, Loos M, Heldring CM, Jurkowski W, van Nierop P, Paliukhovich I, Li KW, del Sol A, Smit AB, Spijker S, van Kesteren RE, Hippocampal extracellular matrix levels and stochasticity in synaptic protein expression increase with age and are associated with age-dependent cognitive decline. Mol Cell Proteomics, 13(11):2975-85(2014) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: Hippocampus, Synaptosome, Natural aging, Mouse, iTRAQ

Dr. Ronald van Kesteren
contact affiliation	Center for Neurogenomics and Cognitive Research, VU Amsterdam De Boelelaan 1085 1081 HV Amsterdam
contact email	ronald.van.kesteren@cncr.vu.nl
lab head
Pim van Nierop
contact affiliation	Molecular and Cellular Neurobiology
contact email	pim.van.nierop@cncr.vu.nl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001135
     1. Label: PRIDE project
     2. Name: Relative protein quantification in mouse hippocampal synaptosomes during aging series