PXD001132

PXD001132 is an original dataset announced via ProteomeXchange.

Title	Soybean Symbiosome Membrane Proteome
Description	Symbiosome membranes (SM) and symbiosome space (SS) fractions were isolated from nitrogen fixing soybean root nodules and analyzed using non-gel proteomic techniques. Bicarbonate stripping and chloroform-methanol extraction of isolated SM were used to enrich for hydrophobic integral membrane proteins.. One-hundred and seventy-two proteins were identified as components of the SM, with an additional fifteen proteins identified from peripheral membrane and SS protein fractions. Proteins involved in a range of cellular processes such as metabolism, protein folding and targeting, signalling and transport were identified. These included a number of proteins previously localized to the SM, such as nodulin 26, an aquaglyceroporin. Transport classes identified include sulphate, calcium, peptide/dicarboxylate, nitrate and metal ion transporters. This proteome will provide a rich resource for the study of the legume-rhizobium symbiosis.
HostingRepository	PRIDE
AnnounceDate	2015-08-12
AnnouncementXML	Submission_2015-08-12_02:03:34.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001132
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Victoria Clarke
SpeciesList	scientific name: Glycine max (Soybean) (Glycine hispida); NCBI TaxID: 3847;
ModificationList	Oxidation; Carboxymethyl
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-14 06:06:17	ID requested
⏵ 1	2015-08-12 02:03:35	announced

Clarke VC, Loughlin PC, Gavrin A, Chen C, Brear EM, Day DA, Smith PM, Proteomic analysis of the soybean symbiosome identifies new symbiotic proteins. Mol Cell Proteomics, 14(5):1301-22(2015) [pubmed]

curator keyword: Biological

submitter keyword: Soybean, symbiosome, membrane, legume

Penny Smith
contact affiliation	University of Sydney
contact email	penny.smith@sydney.edu.au
lab head
Victoria Clarke
contact affiliation	Macquarie University
contact email	tory.clarke@mq.edu.au
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001132
     1. Label: PRIDE project
     2. Name: Soybean Symbiosome Membrane Proteome