PXD001130

PXD001130 is an original dataset announced via ProteomeXchange.

Title	Proteomics of GPI-anchored proteins
Description	GPI-anchored proteins (GPI-APs) are an important class of glycoproteins that are tethered to the surface of mammalian cells via the lipid glycosylphosphatidylinositol (GPI). GPI-APs have been implicated in many important cellular functions including cell adhesion, cell signaling, and immune regulation. Proteomic identification of mammalian GPI-APs en masse has been limited technically by poor sensitivity for these low abundance proteins and the use of methods that destroys cell integrity. Here we present methodology that permits identification of GPI-APs liberated directly from the surface of intact mammalian cells through exploitation of their appended glycans to enrich for these proteins ahead of LC-MS/MS analyses. We validate our approach in HeLa cells, identifying a greater number of GPI-APs from intact cells than has been previously identified from isolated HeLa membranes and a lipid raft preparation. We further apply our approach to define the cohort of endogenous GPI-APs that populate the distinct apical and basolateral membrane surfaces of polarized epithelial cell monolayers. Our approach provides a new method to achieve greater sensitivity in the identification of low abundance GPI-APs from the surface of live cells and the non-destructive nature of the method provides new opportunities for the temporal or spatial analysis of cellular GPI-AP expression and dynamics.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_04:05:24.202.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Manesh Shah
SpeciesList	scientific name: Canis familiaris (Dog) (Canis lupus familiaris); NCBI TaxID: 9615;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-14 00:34:16	ID requested
1	2016-07-08 06:37:35	announced
⏵ 2	2024-10-22 04:05:32	announced	2024-10-22: Updated project metadata.

Cortes LK, Vainauskas S, Dai N, McClung CM, Shah M, Benner JS, Corr, ê, a IR, VerBerkmoes NC, Taron CH, Proteomic identification of mammalian cell surface derived glycosylphosphatidylinositol-anchored proteins through selective glycan enrichment. Proteomics, 14(21-22):2471-84(2014) [pubmed]

10.1002/pmic.201400148;

curator keyword: Biological

submitter keyword: proteomics GPI proteins mammalian glycan glycosylphosphatidylinositol

Dr. Christopher Taron
contact affiliation	New England BioLabs Inc
contact email	taron@neb.com
lab head
Manesh Shah
contact affiliation	New England BioLabs Inc
contact email	shahmaneshb@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2016/07/PXD001130

PRIDE project URI

• PRIDE
  1. PXD001130
     1. Label: PRIDE project
     2. Name: Proteomics of GPI-anchored proteins