PXD001129

PXD001129 is an original dataset announced via ProteomeXchange.

Title	Dissection of IL-2 and IL-15 signaling pathways
Description	The aim of this study was to compare the signaling cascades initiated by the two closely related cytokines IL-2 and IL-15. Considering the relevance of protein tyrosine phosphorylation in signal transduction, in order to quantify changes in protein phosphorylation in response to IL-2 and IL-15, we combined triple SILAClabeling of leukemic T-cells with phosphotyrosine (pY)-specific antibody-based protein enrichment followed by mass spectrometry (MS) analysis. Following the strategy described above, we managed to decipher in detail the complex signaling networks triggered by IL-2 and IL-15. Interestingly, a large number of components of the three main signaling pathways known to be initiated in response to the interleukins (JAK/STAT; RAS/MAPK; PI3K/AKT) were identified.
HostingRepository	PRIDE
AnnounceDate	2014-09-23
AnnouncementXML	Submission_2014-09-23_07:41:45.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nerea Osinalde
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-11 03:23:51	ID requested
⏵ 1	2014-09-23 07:41:46	announced

Osinalde N, Sanchez-Quiles V, Akimov V, Guerra B, Blagoev B, Kratchmarova I, Simultaneous dissection and comparison of IL-2 and IL-15 signaling pathways by global quantitative phosphoproteomics. Proteomics, 15(2-3):520-31(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: SILAC, signaling, phosphotyrosine, IL-2, IL-15

Nerea Osinalde
contact affiliation	Department of Biochemistry and Molecular Biology, Center of Experimental BioInformatic, University of Southern Denmark
contact email	nmo@bmb.sdu.dk
lab head
Nerea Osinalde
contact affiliation	Postdoc
contact email	nmo@bmb.sdu.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001129
     1. Label: PRIDE project
     2. Name: Dissection of IL-2 and IL-15 signaling pathways