PXD001117

PXD001117 is an original dataset announced via ProteomeXchange.

Title	Human Cardiac Stem Cells Receptome
Description	Human cardiac stem cells (hCSC) express a portfolio of plasma membrane receptors that are involved in the regulatory auto/paracrine feedback loop mechanism of activation of these cells, and consequently contribute to myocardial regeneration. In order to attain a comprehensive description of hCSC receptome and overcoming the inability demonstrated by other technologies applied in receptor identification, mainly due to the transmembrane nature, high hydrophobic character and relative low concentration of these proteins, we have exploited and improved a proteomics workflow. This approach was based on the enrichment of hCSC plasma membrane fraction and addition of pre-fractionation steps prior to MS analysis. More than one hundred plasma membrane receptors were identified. The data reported herein constitute a valuable source of information to further understand cardiac stem cells activation mechanisms and the subsequent cardiac repair process.
HostingRepository	PRIDE
AnnounceDate	2015-01-30
AnnouncementXML	Submission_2015-01-30_05:08:57.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Patrícia Gomes-Alves
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-09 01:21:24	ID requested
⏵ 1	2015-01-30 05:08:58	announced

Gomes-Alves P, Serra M, Brito C, R-Borlado L, L, ó, pez JA, V, á, zquez J, Carrondo MJ, Bernad A, Alves PM, Exploring analytical proteomics platforms toward the definition of human cardiac stem cells receptome. Proteomics, 15(7):1332-7(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: Human Cardiac Stem Cells, nanoLC-MS/MS, Pre-fractionation, Proteomics, Receptome

Paula M. Alves
contact affiliation	CEO, IBET, Apartado 12, 2781-901 Oeiras, Portugal Director Animal Cell Technology Unit, ITQB-UNL Inv Associate Professor, FCT-UNL
contact email	marques@ibet.pt
lab head
Patrícia Gomes-Alves
contact affiliation	iBET
contact email	palves@ibet.pt
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/01/PXD001117

PRIDE project URI

• PRIDE
  1. PXD001117
     1. Label: PRIDE project
     2. Name: Human Cardiac Stem Cells Receptome