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PXD001115

PXD001115 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleProteomics of NGF-TrkA signaling dynamics in neuroblastoma cells
DescriptionWe used quantitative mass spectrometry-based proteomics to unravel global nerve growth factor (NGF)-induced TrkA signaling dynamics at the interactome, phosphoproteome and proteome level. A tetracycline-inducible system for TrkA expression was generated in the human neuroblastoma cell line, SH-SY5Y. TrkA-induced cells were stimulated with NGF for different time points to follow phosphoproteome, interactome and proteome changes on a temporal scale. In a triple SILAC setup (Light: Lys0,Arg0; Medium: Lys4,Arg6; and Heavy: Lys8,Arg10), the samples were stimulated with NGF as indicated. Phosphoproteome: 0, 10, 45 min and 0, 120, 120 min+cycloheximide. Interactome: 0, 5, 10 min. Proteome: 0, 24, 48 h. All experiments were performed as biological replicates.
HostingRepositoryPRIDE
AnnounceDate2015-05-06
AnnouncementXMLSubmission_2015-05-06_07:44:40.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterKristina Bennet Emdal
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListphosphorylated residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-07-08 07:10:46ID requested
12015-05-06 07:44:41announced
Publication List
Emdal KB, Pedersen AK, Bekker-Jensen DB, Tsafou KP, Horn H, Lindner S, Schulte JH, Eggert A, Jensen LJ, Francavilla C, Olsen JV, Temporal proteomics of NGF-TrkA signaling identifies an inhibitory role for the E3 ligase Cbl-b in neuroblastoma cell differentiation. Sci Signal, 8(374):ra40(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: NGF, TrkA, neuroblastoma, mass spectrometry, SILAC, phosphoproteome, interactome, proteome
Contact List
Jesper V. Olsen
contact affiliationNovo Nordisk Foundation Center for Protein Research Faculty of Health & Medical Sciences University of Copenhagen Blegdamsvej 3B DK-2200 Copenhagen N Denmark
contact emailjesper.olsen@cpr.ku.dk
lab head
Kristina Bennet Emdal
contact affiliationNNF Center for Protein Research, Proteomics Program, Faculty of Health and Medical Sciences, University of Copenhagen.
contact emailkristina.emdal@cpr.ku.dk
dataset submitter
Full Dataset Link List
Dataset FTP location
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