PXD001109

PXD001109 is an original dataset announced via ProteomeXchange.

Title	Shotgun analysis of plasma fibrin clot-bound proteins in patients with acute myocardial infarction
Description	The presence and amount of the proteins within a plasma clot may influence clot properties, like susceptibility to fibrinolysis, however, the plasma clot proteome has not yet been extensively described. The aim of the study was to investigate the protein composition of clots prepared ex vivo from plasma of the peripheral blood of four patients with acute myocardial infarction (AMI) in two time points: in the acute ischemic phase and two months later during the standard therapy. Proteomic analysis revealed a total number of 62 proteins identified in all 8 samples grouping into several distinct functional clusters (e.g. cholesterol transporter activity, immunoglobulin binding and peptidase regulatory activity). The protein signatures of clots differed significantly depending on time after ACS, showing 30% greater variability in protein composition of the clots prepared in the plasma two months after the onset of AMI as compared to the clots generated at the time of admission to the hospital. Several proteins that could be involved in clot formation and resolution showed an interesting pattern of changes over time. For example α2-antiplasmin, which was robustly present in clots of all patients in acute phase of AMI, was detectable in lower abundance in only 2 clots prepared in plasma taken 8-12 weeks after AMI, whereas serotransferrin can be detected only in the clots prepared from plasma taken later during the therapy. In conclusion, we provided the first qualitative analysis of proteomes of fibrin clots generated ex vivo in plasma taken from patients with AMI showing differences in protein composition between clots generated in the acute ischemic phase and those prepared two months later. It might be hypothesized that differences involving several proteins of potential influence on within-clot fibrinolysis and clot stability may partially explain time-dependent changes in the clots structure and firmness in patients with AMI.
HostingRepository	PRIDE
AnnounceDate	2017-10-24
AnnouncementXML	Submission_2017-10-24_05:51:34.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Maciej Suski
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Velos Plus

Revision	Datetime	Status	ChangeLog Entry
0	2014-07-04 01:28:44	ID requested
1	2016-12-08 02:02:54	announced
⏵ 2	2017-10-24 05:51:35	announced	Updated project metadata.

Suski M, Siudut J, Z, ą, bczyk M, Korbut R, Olszanecki R, Undas A, Shotgun analysis of plasma fibrin clot-bound proteins in patients with acute myocardial infarction. Thromb Res, 135(4):754-9(2015) [pubmed]

curator keyword: Cardiovascular, Biomedical

submitter keyword: acute coronary syndromes, fibrin, clot, shotgun proteomics

Rafał Olszanecki
contact affiliation	Jagiellonian University Medical College
contact email	mfolszan@cyf-kr.edu.pl
lab head
Maciej Suski
contact affiliation	Chair of Pharmacology
contact email	maciej.suski@uj.edu.pl
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001109
     1. Label: PRIDE project
     2. Name: Shotgun analysis of plasma fibrin clot-bound proteins in patients with acute myocardial infarction