PXD001100

PXD001100 is an original dataset announced via ProteomeXchange.

Title	Human Sumoylome (SUMO-2) upon bacterial infection
Description	Given the role of SUMOylation in pathogenic infection, we wanted to evaluate the changes to the host SUMO-2 subproteome during Shigella infection. HeLa cells overexpressing TAP-SUMO-2 or TAP-empty were used in conjunction with SILAC (Stable Isotope Labeling of Amino acids in Culture) (Golebiowski et al, 2009, 2010). Cells were grown in Dulbecco’s modified Eagle’s medium except that L-arginine and L-lysine were replaced with stable isotope (SILAC) forms depending on the treatment. Medium was supplemented with 10% dialyzed fetal calf serum. SILAC experiment compared TAP-containing cells (Lys0 and Arg0) with invasive Shigella flexneri (M90T) infected TAP-SUMO-2-containing cells (4,4,5,5-D4-lysine, Lys4, and 13C6- arginine, Arg6) and TAP-SUMO-2-containing cells infected with (mxiD) non-invasive strain of Shigella (13C6 15N2-lysine, Lys8, and 13C6 15N4 -arginine, Arg10).
HostingRepository	PRIDE
AnnounceDate	2018-10-19
AnnouncementXML	Submission_2018-10-19_01:27:46.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sabrina Fritah
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-30 07:01:25	ID requested
⏵ 1	2018-10-19 01:27:47	announced

Fritah S, Lhocine N, Golebiowski F, Mounier J, Andrieux A, Jouvion G, Hay RT, Sansonetti P, Dejean A, Sumoylation controls host anti-bacterial response to the gut invasive pathogen Shigella flexneri. EMBO Rep, 15(9):965-72(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Human, HeLa, TAP TAG, SUMO-2, Shigella flexneri

Anne Dejean
contact affiliation	Institut Pasteur, Laboratory of Nuclear Organization and Oncogenesis, INSERM, U993, F-75015 Paris, France
contact email	anne.dejean@pasteur.fr
lab head
Sabrina Fritah
contact affiliation	CRP-Sante
contact email	sabrina.fritah@crp-sante.lu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2018/10/PXD001100

PRIDE project URI

• PRIDE
  1. PXD001100
     1. Label: PRIDE project
     2. Name: Human Sumoylome (SUMO-2) upon bacterial infection