PXD001099

PXD001099 is an original dataset announced via ProteomeXchange.

Title	Piezo1 integration of vascular architecture with physiological force LC-MSMS
Description	The mechanisms by which physical forces regulate cells to determine complexities of vascular structure and function are enigmatic. Here we show the role the ion channel subunit Piezo1 (FAM38A). Disruption of mouse Piezo1 gene disturbed vascular development and was embryonic lethal within days of the heart beating to cause blood flow. Importance of Piezo channels as sensors of blood flow was indicated by Piezo1 dependence of shear stress-evoked ionic current and calcium influx in endothelial cells and the ability of exogenous Piezo1 to confer shear stress sensitivity on cells that otherwise lacked. Downstream of this calcium influx was proteoase activity and spatial organization of endothelial cells to the polarity of the applied force. Without Piezo1, normal endothelial cell organization was lacking. The data suggest Piezo1 channels as pivotal integrators of vascular architacture with physiological mechanical force.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-11-10_03:47:15.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001099
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Beech
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide - site C: 57.021464; acetylated residue: 42.010565; 2-pyrrolidone-5-carboxylic acid (Gln): -17.026549; monohydroxylated residue: 15.994915; L-methionine sulfone: 31.989829; phosphorylated residue: 79.966331
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-30 02:28:24	ID requested
1	2014-08-08 08:04:29	announced
2	2014-10-23 04:59:59	announced	Updated project metadata.
⏵ 3	2014-11-10 03:47:17	announced	Updated project metadata.

Li J, Hou B, Tumova S, Muraki K, Bruns A, Ludlow MJ, Sedo A, Hyman AJ, McKeown L, Young RS, Yuldasheva NY, Majeed Y, Wilson LA, Rode B, Bailey MA, Kim HR, Fu Z, Carter DA, Bilton J, Imrie H, Ajuh P, Dear TN, Cubbon RM, Kearney MT, Prasad RK, Evans PC, Ainscough JF, Beech DJ, Piezo1 integration of vascular architecture with physiological force. Nature, 515(7526):279-282(2014) [pubmed]

curator keyword: Biomedical

submitter keyword: Piezo1, human, mouse, shear stress, label-free, phosphoproteomics, HUVEC, vascular architecture

David J. Beech
contact affiliation	School of Medicine and Multidisciplinary Cardiovascular Research Centre University of Leeds United Kingdom
contact email	D.J.Beech@leeds.ac.uk
lab head
David Beech
contact affiliation	University of Leeds
contact email	D.J.Beech@leeds.ac.uk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001099

PRIDE project URI

• PRIDE
  1. PXD001099
     1. Label: PRIDE project
     2. Name: Piezo1 integration of vascular architecture with physiological force LC-MSMS