PXD001096

PXD001096 is an original dataset announced via ProteomeXchange.

Title	Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment
Description	Mammalian fertilization requires a complex interplay between a sperm cell and an oocyte. A significant proportion of these functions are mediated through the proteomic composition of the sperm surface. These proteomic constituents can present as biomarkers to control and regulate breeding of agricultural animals. Previous studies have addressed the composition of bovine sperm cell apical plasma membrane proteome with nitrogen cavitation enrichment. Alternative workflows would enable to expand the compositional data more globally around the entire sperm’s surface. We used a cell-surface biotin-labelling in combination with differential centrifugation to enrich bovine sperm surface proteins. Using nano-LC MS/MS, 338 proteins were confidently identified in the plasma membrane enriched proteome. These results were bioinformatically analysed to assess the purification and to highlight the potential protein-protein interaction networks on the sperm cell surface. A highly significant degree of enrichment was found for transmembrane and plasma membrane proteins, among them proteins previously not described on bovine sperm (CPQ, CD58, CKLF, CPLV, GLB1L3 and LPCAT2B). Functional categories of sperm-egg interaction, protein turnover, metabolism as well as molecular transport, spermatogenesis and signal transduction were represented by proteins with high quantitative signal in our study. A descriptive overview of the bovine sperm plasma membrane integral and peripheral proteins is provided to complement future studies on animal reproduction and its relation to sperm cell surface.
HostingRepository	PRIDE
AnnounceDate	2015-03-19
AnnouncementXML	Submission_2015-03-19_02:01:51.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Sergo Kasvandik
SpeciesList	scientific name: Bos taurus (Bovine); NCBI TaxID: 9913;
ModificationList	biotinylated residue; deamidated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-30 02:02:24	ID requested
⏵ 1	2015-03-19 02:01:53	announced

Kasvandik S, Sillaste G, Velthut-Meikas A, Mikelsaar AV, Hallap T, Padrik P, Tenson T, Jaakma Ü, K, õ, ks S, Salumets A, Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment. Proteomics, 15(11):1906-20(2015) [pubmed]

curator keyword: Biological

submitter keyword: biotinylation, Bos taurus, plasma membrane proteome, reproductive biology, sperm cell

Andres Salumets
contact affiliation	Competence Centre on Reproductive Medicine and Biology, Tiigi 61b, Tartu, Estonia Department of Obstetrics and Gynaecology, University of Tartu, Puusepa 8, Tartu, Estonia Institute of Bio- and Translational Medicine, University of Tartu, Ravila 19, Tartu, Estonia
contact email	andres.salumets@ccrmb.ee
lab head
Sergo Kasvandik
contact affiliation	University of Tartu, Proteomics core facility
contact email	sergo.kasvandik@ut.ee
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/03/PXD001096

PRIDE project URI

• PRIDE
  1. PXD001096
     1. Label: PRIDE project
     2. Name: Bovine sperm plasma membrane proteomics through biotinylation and subcellular enrichment