PXD001087

PXD001087 is an original dataset announced via ProteomeXchange.

Title	Breast Cancer .
Description	Endocrine resistance in breast cancer is a major clinical problem with poorly understood mechanisms. Mass spectrometry-based proteomics of a clinically-relevant tamoxifen-resistant cell line model identified increased levels of minichromosome maintenance proteins (MCM), including MCM3, as central in cell cycle and DNA replication protein-protein interaction networks associated with tamoxifen resistance. Lowering MCM3 protein expression in tamoxifen-resistant cells restored tamoxifen sensitivity and altered phosphorylation of several cell cycle regulators, such as p53(Ser315, 33), CHK1(Ser317) and cdc25b(Ser323), suggesting that MCM3 activation of important cell cycle-associated proteins overcomes tamoxifen’s anti-proliferative effects. High MCM3 expression in primary tumor tissue from two independent cohorts of ER+ breast cancer patients receiving adjuvant tamoxifen mono-therapy was an independent prognostic marker significantly associated with a shorter recurrence-free survival.
HostingRepository	PRIDE
AnnounceDate	2024-10-03
AnnouncementXML	Submission_2024-10-03_07:58:12.892.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jens Andersen
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-25 01:51:26	ID requested
⏵ 1	2024-10-03 07:58:13	announced

10.1038/s41523-020-00210-8;

L, ø, kkegaard S, Elias D, Alves CL, Bennetzen MV, L, æ, nkholm AV, Bak M, Gjerstorff MF, Johansen LE, Vever H, Bjerre C, Kirkegaard T, Nordenskj, ö, ld B, Fornander T, St, å, l O, Lindstr, ö, m LS, Esserman LJ, Lykkesfeldt AE, Andersen JS, Leth-Larsen R, Ditzel HJ, MCM3 upregulation confers endocrine resistance in breast cancer and is a predictive marker of diminished tamoxifen benefit. NPJ Breast Cancer, 7(1):2(2021) [pubmed]

curator keyword: Biomedical

submitter keyword: NDRG1, tamoxifen resistance,Estrogen receptor-positive breast cancer, cell cycle, minichromosome maintenance proteins (MCM), DNA replication

Henrik J. Ditzel
contact affiliation	Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, DK-5000 Odense C, Denmark
contact email	hditzel@health.sdu.dk
lab head
Jens Andersen
contact affiliation	University of Southern Denmark, Department og Biochemistry and Molecular Biology
contact email	jens.andersen@bmb.sdu.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD001087

PRIDE project URI

• PRIDE
  1. PXD001087
     1. Label: PRIDE project
     2. Name: Breast Cancer