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PXD001087

PXD001087 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleBreast Cancer .
DescriptionEndocrine resistance in breast cancer is a major clinical problem with poorly understood mechanisms. Mass spectrometry-based proteomics of a clinically-relevant tamoxifen-resistant cell line model identified increased levels of minichromosome maintenance proteins (MCM), including MCM3, as central in cell cycle and DNA replication protein-protein interaction networks associated with tamoxifen resistance. Lowering MCM3 protein expression in tamoxifen-resistant cells restored tamoxifen sensitivity and altered phosphorylation of several cell cycle regulators, such as p53(Ser315, 33), CHK1(Ser317) and cdc25b(Ser323), suggesting that MCM3 activation of important cell cycle-associated proteins overcomes tamoxifen’s anti-proliferative effects. High MCM3 expression in primary tumor tissue from two independent cohorts of ER+ breast cancer patients receiving adjuvant tamoxifen mono-therapy was an independent prognostic marker significantly associated with a shorter recurrence-free survival.
HostingRepositoryPRIDE
AnnounceDate2024-10-03
AnnouncementXMLSubmission_2024-10-03_07:58:12.892.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJens Andersen
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-06-25 01:51:26ID requested
12024-10-03 07:58:13announced
Publication List
10.1038/s41523-020-00210-8;
L, ø, kkegaard S, Elias D, Alves CL, Bennetzen MV, L, æ, nkholm AV, Bak M, Gjerstorff MF, Johansen LE, Vever H, Bjerre C, Kirkegaard T, Nordenskj, ö, ld B, Fornander T, St, å, l O, Lindstr, ö, m LS, Esserman LJ, Lykkesfeldt AE, Andersen JS, Leth-Larsen R, Ditzel HJ, MCM3 upregulation confers endocrine resistance in breast cancer and is a predictive marker of diminished tamoxifen benefit. NPJ Breast Cancer, 7(1):2(2021) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: NDRG1, tamoxifen resistance,Estrogen receptor-positive breast cancer, cell cycle, minichromosome maintenance proteins (MCM), DNA replication
Contact List
Henrik J. Ditzel
contact affiliationDepartment of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, DK-5000 Odense C, Denmark
contact emailhditzel@health.sdu.dk
lab head
Jens Andersen
contact affiliationUniversity of Southern Denmark, Department og Biochemistry and Molecular Biology
contact emailjens.andersen@bmb.sdu.dk
dataset submitter
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Dataset FTP location
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