PXD001082

PXD001082 is an original dataset announced via ProteomeXchange.

Title	Quantitative comparison of CSF proteins from T. b. gambiense and T. b. rhodesiense HAT patients
Description	The host central nervous system (CNS) response to infection with Trypanosoma brucei (T. b.) gambiense or T. b. rhodesiense parasites, the causing agent of human African trypanosomiasis (HAT), is a poorly explored area. The two parasites are responsible for respectively a chronic and an acute form of HAT. In both cases, however, the disease progresses from a haemolymphatic first stage (S1) to a meningo-encephalitic second stage (S2) due to the penetration of parasites into the CNS. In the present study, we investigated and compared the cerebrospinal fluid (CSF) from S2 patients affected by either T. b. gambiense or T. b. rhodesiense HAT, using a mass spectrometry quantitative proteomics approach. Gene ontology and pathway analyses on the 222 quantified proteins, revealed a predominant activation of the innate immune and the acute phase responses in rhodesiense HAT. These results were further confirmed through the verification of the over-expression of two proteins involved in these mechanisms, C-reactive protein (CRP) and orosomucoid 1 (ORM1), in 126 S2 HAT patients suffering from either the chronic or the acute form of HAT. Both proteins were significantly increased (p<0.0001) in the CSF of rhodesiense HAT patients. These findings contribute in better understanding the pathophysiological mechanisms of late stage HAT caused by T. b. gambiense or T. b. rhodesiense and pave the way for further investigations on the clinical significance of CRP and ORM1.
HostingRepository	PRIDE
AnnounceDate	2015-01-23
AnnouncementXML	Submission_2015-01-23_01:28:02.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Natalia Tiberti
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue; TMT6plex-126 reporter+balance reagent acylated residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-24 02:10:39	ID requested
⏵ 1	2015-01-23 01:28:02	announced

Dataset with its publication pending

curator keyword: Biomedical

submitter keyword: Human cerebrospinal fluid, human African trypanosomiasis, Tandem Mass Tag (TMT), ESI LTQ Orbitrap

Jean-Charles Sanchez
contact affiliation	Translational Biomarker Group, Department of Human Protein Sciences, University of Geneva, Geneva, Switzerland
contact email	Jean-Charles.Sanchez@unige.ch
lab head
Natalia Tiberti
contact affiliation	Translational Biomarker Group, Department of Human Protein Sciences, University of Geneva, Geneva, Switzerland
contact email	natalia.tiberti@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001082
     1. Label: PRIDE project
     2. Name: Quantitative comparison of CSF proteins from T. b. gambiense and T. b. rhodesiense HAT patients