PXD001081

PXD001081 is an original dataset announced via ProteomeXchange.

Title	Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells
Description	Pancreatic beta cells have well-developed endoplasmic reticulum (ER) to accommodate for the massive production and secretion of insulin. ER homeostasis is vital for normal beta cell function. Perturbation of ER homeostasis contributes to beta cell dysfunction in both type 1 and type 2 diabetes. To systematically identify the molecular machinery responsible for proinsulin biogenesis and maintenance of beta cell ER homeostasis, a widely used mouse pancreatic beta cell line, MIN6 cell was used to purify rough ER. Two different purification schemes were utilized. In each experiment, the ER pellets were solubilized and analyzed by one dimensional SDS-PAGE coupled with HPLC-MS/MS. A total of 1467 proteins were identified in three experiments with ≥95% confidence, among which 1117 proteins were found in at least two separate experiments. Gene ontology analysis revealed a comprehensive profile of known and novel players responsible for proinsulin biogenesis and ER homeostasis. This dataset defines a molecular environment in the ER for proinsulin synthesis, folding and export and laid a solid foundation for further characterizations of altered ER homeostasis under diabetes-causing conditions.
HostingRepository	PRIDE
AnnounceDate	2015-04-15
AnnouncementXML	Submission_2015-04-15_02:34:24.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001081
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Xuequn Chen
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	QSTAR

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-24 02:09:34	ID requested
⏵ 1	2015-04-15 02:34:25	announced

Lee JS, Wu Y, Schnepp P, Fang J, Zhang X, Karnovsky A, Woods J, Stemmer PM, Liu M, Zhang K, Chen X, Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells. Proteomics, 15(9):1508-11(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: insulin biogenesis, GeLC-MS/MS, pancreatic beta cells, endoplasmic reticulum

Xuequn Chen
contact affiliation	Department of Physiology, School of Medicine, Wayne State University
contact email	xchen@med.wayne.edu
lab head
Xuequn Chen
contact affiliation	Wayne State University, Detriot, MI, USA
contact email	xchen@med.wayne.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001081
     1. Label: PRIDE project
     2. Name: Proteomics analysis of rough endoplasmic reticulum in pancreatic beta cells