PXD001080

PXD001080 is an original dataset announced via ProteomeXchange.

Title	Entamoeba histolytica splicing/mRNP proteome
Description	First, the snRNP component U1A was HA-tagged and the intron of the RabX13 gene was MS2-tagged and amoeba transformats were established with such constructs. Next, amoeba transformants were UV cross-linked and their nucler extracts were immunoprecipitated (IP) with anti-HA agarose or MS2-spharose beads. Precipitates were subjected to tandem mass spectrometry (MS/MS) analyses. MS2-IP helped to discriminate the nuclear roles (chromatin-, co-transcriptional-, splicing-related), of the proteins probed.
HostingRepository	PRIDE
AnnounceDate	2014-09-11
AnnouncementXML	Submission_2014-09-11_06:32:19.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Robert Winkler
SpeciesList	scientific name: Entamoeba histolytica; NCBI TaxID: 5759;
ModificationList	monohydroxylated residue; acetylated residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-23 01:43:08	ID requested
⏵ 1	2014-09-11 06:32:20	announced

Vald, é, s J, Nozaki T, Sato E, Chiba Y, Nakada-Tsukui K, Villegas-Sep, ú, lveda N, Winkler R, Azuara-Liceaga E, Mendoza-Figueroa MS, Watanabe N, Santos HJ, Saito-Nakano Y, Galindo-Rosales JM, Proteomic analysis of Entamoeba histolytica in vivo assembled pre-mRNA splicing complexes. J Proteomics, 111():30-45(2014) [pubmed]

curator keyword: Biological

submitter keyword: Entamoeba histolytica, U1A, CLIP, Thermo Electron Velos Orbitrap ETD

Dr. Jesus Valdes Flores
contact affiliation	CINVESTAV Department of Biochemistry
contact email	jvaldes@cinvestav.mx
lab head
Robert Winkler
contact affiliation	CINVESTAV Unidad Irapuato
contact email	robert.winkler@bioprocess.org
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001080

PRIDE project URI

• PRIDE
  1. PXD001080
     1. Label: PRIDE project
     2. Name: Entamoeba histolytica splicing/mRNP proteome