PXD001080 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Entamoeba histolytica splicing/mRNP proteome |
Description | First, the snRNP component U1A was HA-tagged and the intron of the RabX13 gene was MS2-tagged and amoeba transformats were established with such constructs. Next, amoeba transformants were UV cross-linked and their nucler extracts were immunoprecipitated (IP) with anti-HA agarose or MS2-spharose beads. Precipitates were subjected to tandem mass spectrometry (MS/MS) analyses. MS2-IP helped to discriminate the nuclear roles (chromatin-, co-transcriptional-, splicing-related), of the proteins probed. |
HostingRepository | PRIDE |
AnnounceDate | 2014-09-11 |
AnnouncementXML | Submission_2014-09-11_06:32:19.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Robert Winkler |
SpeciesList | scientific name: Entamoeba histolytica; NCBI TaxID: 5759; |
ModificationList | monohydroxylated residue; acetylated residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-06-23 01:43:08 | ID requested | |
⏵ 1 | 2014-09-11 06:32:20 | announced | |
Publication List
Vald, é, s J, Nozaki T, Sato E, Chiba Y, Nakada-Tsukui K, Villegas-Sep, ú, lveda N, Winkler R, Azuara-Liceaga E, Mendoza-Figueroa MS, Watanabe N, Santos HJ, Saito-Nakano Y, Galindo-Rosales JM, Proteomic analysis of Entamoeba histolytica in vivo assembled pre-mRNA splicing complexes. J Proteomics, 111():30-45(2014) [pubmed] |
Keyword List
curator keyword: Biological |
submitter keyword: Entamoeba histolytica, U1A, CLIP, Thermo Electron Velos Orbitrap ETD |
Contact List
Dr. Jesus Valdes Flores |
contact affiliation | CINVESTAV Department of Biochemistry |
contact email | jvaldes@cinvestav.mx |
lab head | |
Robert Winkler |
contact affiliation | CINVESTAV Unidad Irapuato |
contact email | robert.winkler@bioprocess.org |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/09/PXD001080 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001080
- Label: PRIDE project
- Name: Entamoeba histolytica splicing/mRNP proteome