PXD001079

PXD001079 is an original dataset announced via ProteomeXchange.

Title	Preterm birth increases endogenous human milk peptide release compared to term birth
Description	Hundreds of naturally occurring milk peptides have been found in term human milk. Whether there are differences between the levels of these peptides between term and preterm milk remains unknown. Premature milk is produced before complete maturation of the mammary gland and is under the influence of a different hormonal milieu, which could change enzymatic activity and protein expression within the mammary gland and result in an altered peptide profile. We employed nano-liquid chromatography tandem mass spectrometry to identify naturally occurring peptides in term and premature milks at multiple time-points across lactation and compare the abundances of these peptides. We found that preterm milks produced more unique peptide sequences than term milks on average (359 vs. 286). The peptides identified were searched for overlapping sequences with an in-house database of known functional peptides. Specifically, we found that both term and preterm milks contain peptides overlapping with known sequences with antimicrobial, opioid antagonist and immunomodulatory actions. We also compared the enzymes involved in degradation of both milks via bioinformatic analysis. This analysis reveals that plasmin is more active in preterm milk than term milk.
HostingRepository	PRIDE
AnnounceDate	2016-07-08
AnnouncementXML	Submission_2016-07-08_06:19:57.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001079
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	David Dallas
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	deamidated residue; monohydroxylated residue; acetylated residue; dehydrated residue; phosphorylated residue; deaminated residue
Instrument	6520 Quadrupole Time-of-Flight LC/MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-23 01:34:59	ID requested
⏵ 1	2016-07-08 06:19:58	announced

Dallas DC, Smink CJ, Robinson RC, Tian T, Guerrero A, Parker EA, Smilowitz JT, Hettinga KA, Underwood MA, Lebrilla CB, German JB, Barile D, Endogenous human milk peptide release is greater after preterm birth than term birth. J Nutr, 145(3):425-33(2015) [pubmed]

curator keyword: Biomedical

submitter keyword: human milk, peptidomics

David Dallas
contact affiliation	University of California, Davis
contact email	dcdallas@ucdavis.edu
lab head
David Dallas
contact affiliation	University of California, Davis
contact email	dcdallas@ucdavis.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001079
     1. Label: PRIDE project
     2. Name: Preterm birth increases endogenous human milk peptide release compared to term birth