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PXD001072

PXD001072 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens
DescriptionDesulfotomaculum reducens strain MI-1 is a Gram-positive, sulfate-reducing bacterium also capable of reducing Fe(III). Metal reduction in Gram-positive bacteria is poorly understood. Here, we investigated Fe(III) reduction with lactate, a non-fermentable substrate, as the electron donor. Lactate consumption is concomitant to Fe(III) reduction, but does not support significant growth, suggesting that little energy can be conserved from this process and that it may occur fortuitously. D. reducens can reduce both soluble (Fe(III)-citrate) and insoluble (hydrous ferric oxide, HFO) Fe(III). Because physically inaccessible HFO was not reduced, we concluded that reduction requires direct contact under these experimental conditions. This implies the presence of a surface exposed reductase capable of transferring electrons from the cell to the extracellular electron acceptor. With the goal of identifying candidate Fe(III) reductases, we carried out an investigation of the surface proteome (surfaceome) of D. reducens. Cell surface exposed proteins were extracted by trypsin cell shaving or by lysozyme treatment, and analyzed by liquid chromatography-tandem mass spectrometry.
HostingRepositoryPRIDE
AnnounceDate2014-07-31
AnnouncementXMLSubmission_2014-09-22_05:55:44.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD001072
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterRomain Hamelin
SpeciesList scientific name: Desulfotomaculum reducens (strain MI-1); NCBI TaxID: 349161;
ModificationListOxidation; Phospho; Dehydrated; Ammonia-loss; Acetyl; Carbamidomethyl
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-06-18 02:49:03ID requested
12014-07-31 06:33:15announced
22014-09-22 05:55:45announcedUpdated publication reference for PubMed record(s): 25191310.
Publication List
Dalla Vecchia E, Shao PP, Suvorova E, Chiappe D, Hamelin R, Bernier-Latmani R, Characterization of the surfaceome of the metal-reducing bacterium Desulfotomaculum reducens. Front Microbiol, 5():432(2014) [pubmed]
Keyword List
curator keyword: Biological
submitter keyword: Desulfotomaculum reducens metal-reducing surfaceome
Contact List
Marc Moniatte
contact affiliationEPFL Proteomics Core Facility
contact emailmarc.moniatte@epfl.ch
lab head
Romain Hamelin
contact affiliationPCF
contact emailromain.hamelin@epfl.ch
dataset submitter
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Dataset FTP location
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