PXD001070

PXD001070 is an original dataset announced via ProteomeXchange.

Title	Growth cone localization of the mRNA encoding a chromatin regulator modulates neurite outgrowth
Description	Neurons exploit local mRNA translation and retrograde transport of transcription factors to regulate gene expression in response to signaling events at distal neuronal ends. Whether epigenetic factors could also be involved in such regulation is not known. We report that the mRNA encoding the HMGN5 chromatin binding protein localizes to growth cones of both neuronal-like cells and of hippocampal neurons, where it has the potential to be translated, and that HMGN5 can be retrogradely transported into the nucleus along neurites. Loss of HMGN5 function induces transcriptional changes and impairs neurite outgrowth while HMGN5 overexpression induces neurite outgrowth and chromatin decompaction. Interestingly, control of both neurite outgrowth and chromatin structure is dependent on growth cone localization of Hmgn5 mRNA. Our results provide the first evidence that mRNA localization and local translation might serve as a mechanism to couple the dynamic neuronal outgrowth process with chromatin regulation in the nucleus.
HostingRepository	PRIDE
AnnounceDate	2015-04-17
AnnouncementXML	Submission_2015-04-17_02:19:27.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alex von kriegsheim
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-18 01:50:39	ID requested
⏵ 1	2015-04-17 02:19:28	announced

Moretti F, Rolando C, Winker M, Ivanek R, Rodriguez J, Von Kriegsheim A, Taylor V, Bustin M, Pertz O, Growth Cone Localization of the mRNA Encoding the Chromatin Regulator HMGN5 Modulates Neurite Outgrowth. Mol Cell Biol, 35(11):2035-50(2015) [pubmed]

curator keyword: Biomedical, Biological

submitter keyword: HMGN5, neuronal development , chromatin regulation

Alex von Kriegsheim
contact affiliation	Systems Biology Ireland, UCD
contact email	alex.vonkriegsheim@ucd.ie
lab head
Alex von kriegsheim
contact affiliation	Systems Biology
contact email	alex.vonkriegsheim@ucd.ie
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2015/04/PXD001070

PRIDE project URI

• PRIDE
  1. PXD001070
     1. Label: PRIDE project
     2. Name: Growth cone localization of the mRNA encoding a chromatin regulator modulates neurite outgrowth