PXD001068 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Phosphoproteome dynamics in onset and maintenance of Oncogene-Induced Senescence, proteome dataset |
Description | Expression of the BRAFV600E oncoprotein is known to cause benign lesions, for example melanocytic nevi (moles). In spite of the oncogenic function of mutant BRAF, these lesions are arrested by a cell-autonomous mechanism called Oncogene-Induced Senescence (OIS). Infrequently, nevi can progress to malignant melanoma, through mechanisms that are incompletely understood. To gain more insight into this vital tumor suppression mechanism, we performed a mass spectrometry-based screening of the proteome and phosphoproteome in cycling and senescent cells as well as cells that have abrogated senescence. Proteome analysis of senescent cells revealed the upregulation of established senescence biomarkers, including specific cytokines, but also several proteins not previously associated with senescence, including extracellular matrix-interacting. Using both general and targeted phosphopeptide enrichment by Ti4+-IMAC and phosphotyrosine antibody enrichment, we identified over 15,000 phosphorylation sites. Among the regulated phosphorylation sites we encountered components of the interleukin, BRAF/MAPK and CDK-retinoblastoma (Rb) pathways and several other factors. The extensive proteome and phosphoproteome dataset of BRAFV600E-expressing senescent cells provides molecular clues as to how OIS is initiated, maintained or evaded, serving as a comprehensive proteomic basis for functional validation. |
HostingRepository | PRIDE |
AnnounceDate | 2015-01-15 |
AnnouncementXML | Submission_2015-01-15_03:33:12.xml |
DigitalObjectIdentifier | https://dx.doi.org/10.6019/PXD001068 |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Supported dataset by repository |
PrimarySubmitter | Erik L. de Graaf |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; 2x(13)C: 6x(2)H labeled dimethylated L-arginine; dihydroxylated residue; formylated residue |
Instrument | LTQ Orbitrap Velos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2014-06-17 06:10:31 | ID requested | |
⏵ 1 | 2015-01-15 03:33:13 | announced | |
Publication List
de Graaf EL, Kaplon J, Zhou H, Heck AJ, Peeper DS, Altelaar AF, Phosphoproteome dynamics in onset and maintenance of oncogene-induced senescence. Mol Cell Proteomics, 13(8):2089-100(2014) [pubmed] |
Keyword List
ProteomeXchange project tag: PRIME-XS Project |
curator keyword: Biomedical |
submitter keyword: oncogene-induced senescence, proteome, phosphoproteome, cancer |
Contact List
Albert Heck |
contact affiliation | Utrecht University |
contact email | a.j.r.heck@uu.nl |
lab head | |
Erik L. de Graaf |
contact affiliation | Biomolecular Mass Spectrometry and Proteomics Laboratory, Utrecht University, Utrecht, The Netherlands |
contact email | e.l.degraaf@uu.nl |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD001068
- Label: PRIDE project
- Name: Phosphoproteome dynamics in onset and maintenance of Oncogene-Induced Senescence, proteome dataset