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PXD001066 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleReference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation
DescriptionThe differentiation of human CD4+ T cells into different subtypes of T helper cells and regulatory T cells is crucial for an appropriate immunological response. Among all subtypes Th1 cells are the most prominent specific cell type, representing about 50% of all lymphocytes. So far most global proteomic studies have used either only partially purified T helper cell subpopulations and/or have employed artificial protocols for inducing specific T helper cell subtypes and/or applied gel based approaches. These studies have shed light on molecular details of certain aspects of the proteome. Nevertheless a more global analysis of highly pure primary naïve and Th1 cells by LC-MS/MS is needed in order to contribute to the proteome based T cell subtype characterization. We were able to identify 1757 proteins in total, out of which 49 were significantly regulated. The utilization of highly purified Th1 cells for a global proteome assessment and the bioinformatical comparison to naïve cells reveals the relevance of changes in the metabolism and the ubiquitination pathways upon T cell differentiation.
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterJanina Tomm
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentLTQ Orbitrap Velos
Dataset History
RevisionDatetimeStatusChangeLog Entry
02014-06-16 02:39:42ID requested
12015-10-13 01:42:20announced
Publication List
Pagani M, Rockstroh M, Schuster M, Rossetti G, Moro M, Crosti M, Tomm JM, Reference proteome of highly purified human Th1 cells reveals strong effects on metabolism and protein ubiquitination upon differentiation. Proteomics, 15(21):3644-7(2015) [pubmed]
Keyword List
curator keyword: Biomedical
submitter keyword: human, CD4+, Th1 cells, LC-MS/MS
Contact List
Martin von Bergen
contact affiliationDepartments of Proteomics and Metabolomics Helmholtz Centre for environmental research - UFZ Permoserstrasse 15 04318 Leipzig Germany
contact emailmartin.vonbergen@ufz.de
lab head
Janina Tomm
contact affiliationHelmholtzzentrum für Umweltforschung - UFZ
contact emailjanina.tomm@ufz.de
dataset submitter
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