PXD001063

PXD001063 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of cAMP-mediated signaling during differentiation of 3T3-L1 preadipocytes
Description	Initiation of adipocyte differentiation is promoted by the synergistic action of insulin/insulin-like growth factor, glucocorticoids, and agents activating cAMP-dependent signaling. The action of cAMP is mediated via PKA and Epac, where at least part of the PKA function relates to strong repression of Rho kinase activity, whereas Epac counteracts the reduction in insulin/insulin-like growth factor signaling associated with complete repression of Rho kinase activity. However, detailed knowledge of the Epac-dependent branch and the interplay with PKA is still limited. In the present study, we present a comprehensive evaluation of Epac-mediated processes and their interplay with PKA during the initiation of 3T3-L1 preadipocyte differentiation using a combination of proteomics, molecular approaches and bioinformatics. Proteomic analyses revealed 7 proteins specifically regulated in response to Epac activation, 4 in response to PKA activation, and 11 in response to the combined activation by Epac and PKA during the initial phase of differentiation. Network analyses indicated that the identified proteins are involved in pathways of importance for glucose metabolism, inositol metabolism and calcium-dependent signaling thereby adding a novel facet to our understanding of cAMP-mediated potentiation of adipocyte differentiation.
HostingRepository	PRIDE
AnnounceDate	2014-09-22
AnnouncementXML	Submission_2014-09-22_08:27:08.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD001063
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Kamil Borkowski
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue
Instrument	4800 Proteomics Analyzer

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-16 00:41:20	ID requested
⏵ 1	2014-09-22 08:27:09	announced

Borkowski K, Wrzesinski K, Rogowska-Wrzesinska A, Audouze K, Bakke J, Petersen RK, Haj FG, Madsen L, Kristiansen K, Proteomic analysis of cAMP-mediated signaling during differentiation of 3 T3-L1 preadipocytes. Biochim Biophys Acta, 1844(12):2096-107(2014) [pubmed]

curator keyword: Biological

submitter keyword: cAMP

Epac

adipogenesis

two-dimensional gel electrophoresis

Karsten Kristiansen
contact affiliation	Copenhagen University
contact email	kk@bio.ku.dk
lab head
Kamil Borkowski
contact affiliation	University of Copenhagen
contact email	kamilborkowski@bio.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001063
     1. Label: PRIDE project
     2. Name: Proteomic analysis of cAMP-mediated signaling during differentiation of 3T3-L1 preadipocytes