PXD001058

PXD001058 is an original dataset announced via ProteomeXchange.

Title	Unravelling the proteomic profile of rice meiocytes during early meiosis
Description	Transfer of genetic traits from wild or related species into cultivated rice is nowadays an important aim in rice breeding. Breeders use genetic crosses to introduce desirable genes from exotic germplasms into cultivated rice varieties. However, in many hybrids there is only a low level of pairing (if existing) and recombination at early meiosis between cultivated rice and wild relative chromosomes. With the objective of getting deeper into the knowledge of the proteins involved in early meiosis, when chromosomes associate correctly in pairs and recombine, the proteome of isolated rice meiocytes has been characterized by nLC-MS/MS at every stage of early meiosis (prophase I). Up to 1316 different proteins have been identified in rice isolated meiocytes in early meiosis, being 422 exclusively identified in early prophase I (leptotene, zygotene or pachytene). The classification of proteins in functional groups showed that 167 were related to chromatin structure and remodelling, nucleic acid binding, cell-cycle regulation and cytoskeleton. Moreover, the putative roles of sixteen proteins which have not been previously associated to meiosis or were not identified in rice before, are also discussed namely: seven proteins involved in chromosome structure and remodeling, five regulatory proteins (such as SKP1 (OSK), a putative CDK2 like efector), a protein with RNA recognition motifs, a neddylation-related protein, and two microtubule-related proteins. Revealing the proteins involved in early meiotic processes could provide a valuable tool kit to manipulate chromosome associations during meiosis in rice breeding programs.
HostingRepository	PRIDE
AnnounceDate	2014-10-23
AnnouncementXML	Submission_2014-10-23_03:58:14.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Enriqueta Alos
SpeciesList	scientific name: Rice(O. sativa); NCBI TaxID: 4530;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-12 00:32:32	ID requested
1	2014-08-13 02:57:35	announced
⏵ 2	2014-10-23 03:58:15	announced	Updated project metadata.

Collado-Romero M, Al, ó, s E, Prieto P, Unravelling the proteomic profile of rice meiocytes during early meiosis. Front Plant Sci, 5():356(2014) [pubmed]

curator keyword: Biological

submitter keyword: prophase I, chromosome recognition, chromosome pairing, meiocytes proteome, SKP1 proteins, cytoskeleton, MS/MS

Pilar Prieto
contact affiliation	Instituto de Agricultura Sostenible-CSIC Av. Menéndez Pidal s/n Campus Alameda del Obispo Apartado 4084, 14080 Córdoba, Spain
contact email	pilar.prieto@ias.csic.es
lab head
Enriqueta Alos
contact affiliation	IAS-CSIC
contact email	ealos@ias.csic.es
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2014/08/PXD001058

PRIDE project URI

• PRIDE
  1. PXD001058
     1. Label: PRIDE project
     2. Name: Unravelling the proteomic profile of rice meiocytes during early meiosis