PXD001056

PXD001056 is an original dataset announced via ProteomeXchange.

Title	Label free IMS-MSE protein quantification for plant Golgi protein localisation and abundance
Description	Golgi resident proteins in Arabidopsis thaliana are challenging to quantify since the abundance of this organelle is relatively low within the cell. In this study an organelle fractionation approach, targeting the Golgi apparatus, was combined with a label free quantitative MS, data-independent acquisition (DIA) method employing ion mobility separation known as LC-IMS-MSE, to simultaneously localise proteins to the Golgi apparatus and assess their relative quantity. In total 33 proteins were localised to Golgi apparatus and 102 Golgi localised proteins were quantified.
HostingRepository	PRIDE
AnnounceDate	2016-07-08
AnnouncementXML	Submission_2016-07-08_06:18:21.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Pavel Shliaha
SpeciesList	scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationList	iodoacetamide derivatized residue
Instrument	Synapt MS

Revision	Datetime	Status	ChangeLog Entry
0	2014-06-11 03:11:32	ID requested
⏵ 1	2016-07-08 06:18:22	announced

Nikolovski N, Shliaha PV, Gatto L, Dupree P, Lilley KS, Label-free protein quantification for plant Golgi protein localization and abundance. Plant Physiol, 166(2):1033-43(2014) [pubmed]

curator keyword: Biological

submitter keyword: Arabidopsis thaliana, organelle proteomics, absolute quantitation, LOPIT, LOPIMS, MSE, HDMSE, IMS-MSE, data-independent acquisition

Kathryn Susan Lilley
contact affiliation	Cambridge Centre for Proteomics, Department of Biochemistry, University of Cambridge, UK
contact email	ksl23@cam.ac.uk
lab head
Pavel Shliaha
contact affiliation	Cambridge Center For Proteomics
contact email	pvs22@cam.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD001056
     1. Label: PRIDE project
     2. Name: Label free IMS-MSE protein quantification for plant Golgi protein localisation and abundance